市政污泥生物干化系统酶强化影响

The impact of enzyme enhancement on municipal sludge biological drying system

通过复合酶(蛋白酶和淀粉酶)的添加,探究酶强化对市政污泥生物干化系统有机质降解和高温持续时间的影响机制.研究结果表明,复合酶的添加提高了腐殖质前体及醌基官能团的含量,增强系统的电子转移能力,最终促进了堆体中蛋白质和总糖的高效降解.生物干化系统达到高温期的速率提升3.75倍,最高温由59.2℃提升至72.8℃,高温持续时间(>50℃)提升5.56倍.微生物分析结果发现酶的添加使得厚壁菌门(地芽孢杆菌、脲芽孢杆菌、芽孢杆菌)、Caldibacillus(热杆菌属)等好氧嗜热微生物的丰度增加.结合代谢途径分析结果,复合酶的添加提升了生物干化系统高温期革兰氏阳性菌的丰度,强化了三磷酸腺苷结合盒(ATP-binding cassette)转运蛋白途径,加快了小分子有机质(氨基酸、多肽、碳水化合物等)的膜转运过程和微生物可代谢物质的供给,为市政污泥生物干化系统微生物效率提升提供了更为充分的基质保障.

The effects of enzyme intensification were explored by adding composite enzymes(protease and amylase)to the municipal sludge bio drying system.The addition of these enzymes was found to increase the content of humus precursors and quinone functional groups,enhance the system's electron transfer ability,and promote the efficient degradation of protein and total sugar in the reactor.The rate of reaching high temperature in the bio drying system was increased by 3.75times,with the maximum temperature rising from 59.2℃to 72.8℃,and the duration of high temperature(>50℃)increasing by 5.56times.Microbial analysis results indicated that the addition of enzymes led to an increase in the abundance of aerobic thermophilic microorganisms such as Firmicutes(Geobacillus,Ureibacillus,Bacillus),Caldibacillus(Thermobacillus).The addition of complex enzymes also enhanced the abundance of Gram-positive bacteria in the biological drying system at high temperatures,strengthened the ATP-binding cassette transporter pathway,accelerated the membrane transport process of small molecular organic matter(amino acids,peptides,carbohydrates,etc.),and improved the supply of microbial metabolizable substances,thereby providing a more sufficient substrate guarantee for the improvement of microbial efficiency in the municipal sludge biological drying system.