硼掺杂分子印迹聚合物修饰电化学传感器测定水产品中孔雀石绿

Determination of malachite green in aquatic products by boron doped molecularly imprinted polysiloxanemer modified electrochemical sensor

目的建立基于硼掺杂表面分子印迹聚合物(boron doped molecularly imprinted polysiloxanemer,BMIP)修饰碳糊电极(carbon paste electrode,CPE)构建的电化学传感器测定水产品中孔雀石绿(malachite green,MG)含量的检测方法。方法以苯基三甲氧基硅烷和甲基三甲氧基硅烷为功能单体,MG为模板分子,间氨基苯硼酸为掺杂剂,四乙氧基硅烷为交联剂合成BMIP,并用BMIP对CPE进行修饰,构建BMIP-CPE检测水产品中MG的含量。结果通过优化功能单体、掺杂剂、交联剂用量以及洗脱剂、富集时间等实验参数,在最佳条件下,采用差分脉冲伏安法测得水产品中MG的线性范围为0.2~60.0μmol/L,相关系数为0.9992,检测限为0.075μmol/L,BMIP-CPE具有良好的稳定性和识别选择性,将本方法应用于市售鱼虾等水产品MG的检测,加标回收率为90.2%~97.8%,并与高效液相色谱法进行比较,结果差异无统计学意义(P>0.05)。结论硼掺杂分子印迹聚合物修饰电化学传感器测定水产品中的MG检测方法操作简单快捷,线性范围良好,特异性和准确度高,适用于水产品中MG的测定。

Objective To establish a new method for the determination of malachite green(MG)in aquatic products using the electrochemical sensor that was constructed by modifying carbon paste electrode(CPE)using boron doped molecularly imprinted polysiloxanemer(BMIP).Methods BMIP was synthesized using phenyltrimethoxysilane and methyltrimethoxysilane as functional monomers,MG as template molecule,meta aminophenylboronic acid as dopant,and tetraethoxysilane as crosslinking agent.BMIP was used to modify CPE and construct electrochemical sensor(BMIP-CPE)for detecting MG content in aquatic products.Results By optimizing the dosage of functional monomers,dopants,crosslinking agents,eluents,enrichment time and other experimental parameters,under optimal conditions,the linear range of MG in aquatic products was determined to be 0.2-60.0μmol/L using differential pulse voltammetry,with a correlation coefficient of 0.9992 and a detection limit of 0.075μmol/L.In addition,the BMIP-CPE showed enough stability and good reproducibility.This method was applied to the detection of MG in commercially available aquatic products such as fish and shrimp,with an acceptable recoveries of 90.2%to 97.8%.The results were compared with high performance liquid chromatography,and the difference was not statistically significant(P>0.05).Conclusion The BMIP modified electrochemical sensor is a simple and fast method for detecting MG in aquatic products,with good linear range,high specificity and accuracy,and is suitable for the determination of MG in aquatic products.