樟树籽仁油的急性及42d高剂量经口毒性评价

Evaluation of acute and 42-day high-dose oral toxicity of Cinnamomum camphora seed kernel oil

为评价樟树籽仁油的食用安全性,以ICR小鼠为试验动物,通过急性经口毒性试验确定樟树籽仁油的急性毒性剂量和分级;以SD大鼠为试验动物,进行42 d高剂量(4.0 mL/kg)经口毒性试验评价樟树籽仁油的亚慢性经口毒性。结果表明:ICR小鼠生理和活动均正常,无中毒和死亡现象,其组织器官均没有肉眼可见病变且无剂量-反应关系,樟树籽仁油对ICR小鼠急性经口毒性半数致死量(LD50)大于21.5 g/kg,根据急性毒性剂量分级标准判定樟树籽仁油无急性经口毒性,属实际无毒级;樟树籽仁油对SD大鼠的生长和生理没有影响,对SD大鼠的作用靶器官无毒害作用,表明樟树籽仁油在连续42 d饲喂期内没有呈现出亚慢性经口毒性。综上,樟树籽仁油无急性经口毒性,也无42 d高剂量经口毒性,食用安全性高。

In order to evaluate the edible safety of Cinnamomum camphora seed kernel oil(CCSKO),the acute toxicity dose and classification of CCSKO were determined by acute oral toxicity test with ICR mice as the test animals,and the subchronic oral toxicity of CCSKO was evaluated by 42-day high-dose(4.0 mL/kg)oral toxicity test with SD rats as the test animals.The results showed that the physiology and activity of ICR mice were normal,no poisoning and death phenomenon,no visible lesions in tissues and organs,and no dose-response relationship.The LD50 of acute oral toxicity of CCSKO to ICR mice was greater than 21.5 g/kg.According to the acute toxicity dose grading standard,CCSKO had no acute oral toxicity and was actually non-toxic.The CCSKO had no effect on the growth and physiology of SD rats,and had no toxic effect on the target organs of SD rats,indicating that CCSKO did not show subchronic oral toxicity during the continuous 42 d feeding period.In conclusion,CCSKO does not have acute oral toxicity or 42-day high-dose oral toxicity,and it has high safety for consumption.