高通量测序技术在植物内生菌领域研究中的应用

Application of high-throughput sequencing in research on plant endophytes

植物内生菌是一类能够在植物体内度过部分生命周期而不会引起宿主病害的微生物,是种类和功能十分丰富的微生物资源。随着测序技术的进步,在微生物领域中关于植物内生菌的研究愈发深入。Sanger测序的定向验证性以及较低的测序成本使得该测序技术沿用至今,但其测序的通量较低,不适用于更深层次的内生菌基因组研究。本文简要概述了植物内生菌的研究历程,并综述了以高通量为技术特点的下一代测序(next-generation sequencing,NGS)和以单分子实时测序技术(single-molecule real-time,SMRT)为代表的第三代测序技术在植物内生菌研究领域的应用,总结了不同测序技术在实际研究中的优势和局限性,并探讨了如何根据研究需求选择合适的测序技术,希望为研究者进一步发掘植物内生菌的潜在价值提供参考。

Plant endophytes spend at least part of their life cycle in plants without causing diseases in the hosts,being the microbial resources with rich species and diverse functions.With the advancement in sequencing technology,the microbiological study of endophytes has become increasingly intensive.Being praised for the targeted validation and low cost,Sanger sequencing has been preferred by researchers.However,Sanger sequencing is no longer suitable for deeper genomic study of endophytes due to the low throughput.In this paper,we briefly summarize the research history of endophytes,review the applications of next-generation sequencing characterized by high throughput and third-generation sequencing(single-molecule real-time sequencing)in the research on endophytes,and summarize the research results of different sequencing technologies.Furthermore,we summarize the advantages and limitations of different sequencing technologies and discuss how to choose the appropriate sequencing technology according to the research needs.This review provides a reference for researchers to further explore the potential value of plant endophytes.