姜黄素诱导人肺癌细胞凋亡的实验研究

Experimental Study on the Inducing Apoptosis of Human SPC-A1 Cells by Curcumin

目的探讨姜黄素对人肺腺癌细胞 (SPC A1 )抗癌作用机理。方法采用细胞培养、荧光显微镜、原位末端标记、荧光探测等技术 ,探讨姜黄素抗癌作用及其机理。结果①姜黄素作用于癌细胞后 ,光镜下可见有细胞脱壁 ;荧光镜下可见细胞核破碎 ,细胞裂解成大小不等的凋亡小体 ;原位末端标记法进一步证实 2 0 μM姜黄素作用 2 4h细胞凋亡率达 4 2 .6 7%。②姜黄素作用于癌细胞后 ,使细胞内Ca2 + 、cAMP浓度升高。结论姜黄素可诱导人肺癌细胞凋亡 ,其作用机制可能与细胞内Ca2 + 、cAMP浓度升高有关。

Objective To investigate the mechanism of anti-tumor effects of Curcumin on human SPC-A1 cells.Methods Cell culture,Fluorescence microscope,TUNEL method and Fluorospectrometry technique were used.Results ①By the action of Curcumin,SPC-A1 cells grew against the wall and then peeled off gradually;Fluorescence microscopy showed the nucleus broke into pieces, and cell split into apoptosis bodies in different size;The TUNEL method further certified that apoptotic cells reached 42.67% at the concentration of 20μM Curcumin at 24h.②The level of intracellular free calcium and cAMP concentration in the SPC-A1 cells,treated with Curcumin,was significantly higher than that of the control group.Conclusion Curcumin can induce the apoptosis of SPC-A1 cells, and one of its molecular mechanism may be associated with the rising of the level of intracellular free calcium and cAMP concentration.