同种大鼠带血管异位脾移植模型的建立

A model of vascularized spleen heterotopic allotransplantation in rats

目的 建立同种大鼠带血管异位脾移植的实验模型。方法 远交系Wistar大鼠间行同种大鼠带血管异位脾移植。供脾带门静脉段和腹主动脉段分别与受体的下腔静脉和腹主动脉端—侧吻合 ,供脾异位移植于升结肠旁沟 ,与侧腹壁行三点固定。结果 手术成功率为 69 2 % (18/ 2 6例 ) ,术后 1周存活率为 61 1% (11/ 18例 ) ,2例大鼠获长期存活 (>90d)。脾蒂无一例扭转。组织学检查证实术后早期移植脾存活 ,发生急性排斥反应的时间平均为 3d。

Objective To establish a model of vascularized spleen heterotopic allotransplantation in rats. Methods Vascularized spleen heterotopic allotransplantation was performed among oubred rats. The portal vein and aortic segments of the excised spleen were continuously anastomized end to side to the vena cava and the abdominal aorta, respectively. The graft lain in right paracolic sulcus was fixed to lateral epigastrium. Results The successful rate of operation was 69 2%. The 1 week survival rate was 61 1% postoperatively and 2 rats survived longer than 3 months. None of the rats experienced torsion of the splenic pedicle. Allografts survived in the initial stage and the mean time of acute rejection reaction was 3 days postoperatively. Conclusions It is feasible to establish a model of vasucularized spleen heterotopic allotransplantation in rats.