猪流感病毒广东株的分离鉴定及其特性

Isolation,identification and biological characterization of swine influenza virus Guangdong isolates

对广东省 2 5个猪场有呼吸道症状的 30～ 6 0日龄仔猪 ,用棉拭子采样 ,接种鸡胚分离病毒。经鉴定 ,7株为H1N1亚型毒株。对其中 3株分离株的形态学、部分理化特性和生物学特性的研究结果表明 ,病毒粒子在透射电镜下为杆状、丝状、椭圆形及圆形 ,大小为 80～ 12 0nm ;血凝素(HA)均为热不稳定型 ;均不耐热且对乙醚、氯仿敏感 ;均能凝集公鸡、豚鼠、兔、大白鼠、山羊等动物红细胞 ,对小白鼠红细胞的凝集性有所不同 ,均不凝集牛和猪红细胞 ;经绒毛尿囊腔接种 9日龄鸡胚 ,不能导致鸡胚死亡 ,EID50 分别为 10 — 6 .17/ 0 .2mL、10 - 5.83/ 0 .2mL、10 - 4.4 3/ 0 .2mL ;经鼻腔途径感染小白鼠 ,均未导致小鼠出现症状和死亡 ,接种后第 14d在部分存活的小鼠血清中可检出病毒抗体。

Samples were collected by cottonswabs from 30-60 day old piglets with respiratorysystem problems on 25 farms in Guangdong province. After being inoculated and treated, seven isolates were sent to National Influenza Center in Beijing to identify their subtype. The results showedthat they all belonged toH1N1 subtype of influenza A viruses. Some morphological, physicochemical and biological properties of SGD1, SGD2 and SGD6 isolates were studied. The observations demonstrated that the virions expressed various shapes of spherical, oval, elliptic and elongated forms with diameters in the range of 80-120 nm. Hemagglutinin (HA) of all strains was unstable to heat, and all isolates were heat labile and were sensitive to ether, chloroformand acid. Erythrocytes of cock, guinea pig,rabbit,big white rat, small white mouse andgoatcan be agglomerated by the isolates, while erythrocytes of cattle and pig can not be agglomerated. 9 day old embryos did not die after being inoculated with the viruses via allantoic route, and 50% egg infective doses(EID 50 ) of the 3 strains were 10 -6.17 /0.2mL?10 -5.83 /0.2mL and 10 -4.43 /0.2mL respectively. The small white mice showed no clinical symptoms and no deaths after being inoculated with the viruses via respiratoryroute in our experiment. Serum antibodyHI titers of the survived mice were measuredafter being inoculated14days, some of them had antibody.