薄层扫描法测定仙灵双保心胶囊中人参皂苷Rg_1及人参皂苷Rb_1之和的含量

Content Determination of Sum of Ginsenosides Rg1 and Ginsenosides Rb1 of Xianlingshuangbaoxin Capsule by TLCS

目的:建立仙灵双保心胶囊中人参皂苷Rg_1及人参皂苷Rb_1之和的含量测定方法。方法:双波长薄层扫描法,硅胶G薄层极,展开剂:氯仿-醋酸乙酯-甲醇-水(15:40:22:10)10℃以下放置的下层溶液,显色方法:10％硫酸乙醇溶液。结果:人参皂苷Rg_1线性范围1～5μg,人参皂苷Rb_1线性范围1.07～5.35μg;人参皂苷Rg_1平均回收率为99.8％,RSD为2.6％;人参皂苷Rb_1平均回收率为98.7％,RSD为2.5％。结论:方法准确、可靠,为控制仙灵双保心胶囊的质量提供了科学依据。

Objective: To set up a method for content determination of ginsenosides Rg1 and ginsenosides Rb1 in Xianling-shuangbaoxin capsules. Method: Double-wavelength TLCS, thin layer plate: silicagel plate, developing reagent: chloroform-ethyl ac-etate-methanol-water(15:40:22:10),colorrnetric method: sprayed with 10% USQ) ethanol solution. Result: The linear range: ginsenosides Rg1 from 1-5 μg, ginsenosides Rb1 from 1.07-5.35 μg, a average recovery rate and RSD of ginsenosides Rg1 and ginsenosides Rb1: 99.8%, 2.6% and 98.7%, 2.5% respectively. Conclusion: This method is accurate, reliable and provides a scientic index for controlling the qualities of Xianlingshuangbaoxin capsule.