摘要
目的 :研究Bcl 2、Bax蛋白在CCL4诱导肝纤维化模型大鼠肝组织中的表达以及活血渗湿方对其的作用。方法 :用四氯化碳(CCl4)诱导形成大鼠肝纤维化模型 ,使用活血渗湿方(10g·kg-1d -1生药量灌胃)对该模型肝纤维化大鼠治疗12周 ,设正常对照组、模型对照组和复方鳖甲软肝片阳性对照组 ,用免疫组织化学法(Envition法)检测各组大鼠肝组织Bcl 2、Bax的表达情况。结果 :Bcl 2在正常对照组大鼠肝细胞浆和肝窦呈低水平表达 ,在模型对照组大鼠肝组织分布广泛 ,主要表达在汇管区、纤维间隔、肝窦和肝细胞膜 ,中央静脉和肝细胞浆也有一定表达 ,表达的量明显高于正常对照组(P<0 01)。Bax在正常对照组大鼠的中央静脉及其周围的肝窦呈低水平表达 ,在模型对照组大鼠肝组织主要表达在肝细胞浆 ,其还表达在肝窦、纤维间隔和肝细胞膜中 ,胆管上皮细胞偶见表达 ,表达的量也明显高于正常对照组(P<0 01)。活血渗湿方治疗组大鼠肝组织Bcl 2的表达明显低于模型对照组(P<0 01) ,活血渗湿方治疗组大鼠肝组织Bax的表达也明显低于模型对照组(P<0 05),Bax/Bcl 2无明显差异 ,活血渗湿方对纤维间隔Bax的表达无明显作用 ,能降低Bcl 2的表达 ,调高Bax/Bcl 2。结论 :肝纤维化时Bcl 2和Bax表达加强 ,活血渗湿方能够促进纤维间隔中肝细胞的凋?
Objective:To study the expression of Bclˉ2and Bax in rat hepatic fibrosis model and the role of HuoˉXueˉShenˉShiˉFang(HXSSF).Methods:Hepatic fibrosis was successfully induced in rats by subcutaneous injection of carbon tetrachloride(CCl 4 ),the rats were divided into Fibrosis model group,FuˉFangˉBieˉJiaˉRuanˉGanˉTablet postive control group and HXSSF treatment group(10gkg -1 d -1 ,i.g.for12weeks),normal control group is another group of10rats without any treatment.At last,Bclˉ2and Bax proteins were detected by immunohistochemistry.Results:Bclˉ2was exˉpressed weakly in the plasma of hepatocytes and hepatic sinusoid in normal control,but it was expressed strongly in portal area,fibrous septae,hepatic sinusoid,the plasma and membrane of hepatocytes and central vein in rat fibosis model(P<0.01).Bax was expressed slightly in central vein and the hepatic sinusoid around,it was also expressed strongly in the plasma of hepatocytes,hepatic sinusoid,fibrous septae,membrane of hepatocytes and epithelial cells of bile duct(P<0.01).Compared with that in fibrosis model induced by CCl 4 ,the expression of Bclˉ2was sigˉnificantly lower in rats treated with HXSSF(P<0.01),but it was not significantly different in fibrous septae(P<0.01),the expression of Bclˉ2was significantly lower in rats treated with HXSSF(P<0.05),and it was significantly lower in fibrous septae(P<0.05)too.Conclusion:The expression of Bclˉ2and Bax was increased in rat hepatic fibrosis model.HXSSF can promote the apoptosis of liver cells in fibrous septae,and promoting myofibroblast cells apoptosis is possibly one of the mechanisms of treating hepatic fibrosis.
出处
《浙江中医学院学报》
2003年第2期51-53,共3页
Journal of Zhejiang College of Traditional Chinese Medicine
基金
浙江省自然科学基金(399004)
浙江省教育委员会科研基金(19990350)
浙江省中医药科研基金(2000C10)资助项目