脑缺血再灌注小鼠血小板膜糖蛋白的变化

Changes of Platelet Membrane Glycoprotein in Mice after Cerebral Ischemia-Reperfusion

夹闭小鼠双侧颈总动脉 ,建立脑缺血再灌注动物模型。应用流式细胞术检测再灌注后 3、6、1 2、2 4、48、96h的血小板膜糖蛋白CD31、CD6 1和CD6 2 p的表达。结果显示血小板膜糖蛋白CD31在再灌注 3h时表达增高 ,2 4h达到高峰 ,到 96h仍未降到正常水平。CD6 1在再灌注 3h时表达增高 ,持续到 2 4h,48h时恢复到正常水平。CD6 2 p在再灌注3h时表达增高 ,1 2h达到高峰 ,之后缓慢降低 ,到 96h时仍处较高水平。结论 :脑缺血再灌注可以导致血小板膜糖蛋白CD31、CD6 1、CD6 2p表达量增加 ,可能促进血小板活化。

Cerebral ischemia reperfusion models were established by clamping both common carotid arteries in mice. With the flow cytometry, the expressions of CD 31 ,CD 61 and CD 62p on platelet membrane in model mice were measured at the 3rd h, 6th h, 12th h, 24th h, 48th h, 96th h after reperfusion. The expression of CD 31 on platelet membrane in mice increased at the 3rd h after reperfusion, reached the peak at the 24th h, and then decreased gradually, but the upregulation of CD 31 expression still existed at the 96th h. The expression of CD 61 on platelet membrane in mice increased at the 3rd h after reperfusion, lasted to the 24th h, and then returned to normal level at the 48th h. The expression of CD 62p on platelet membrane in mice increased at the 3rd h after reperfusion, reached the peak at the 12th h, and then decreased gradually, but maintained a higher level at the 96th h. The results indicate cerebral ischemia reperfusion can lead to the upregulation of CD 31 , CD 61 and CD 62p expression on platelet membrane in ischemia reperfusion mice, then may activate the platelets, and result in thrombosis.