非典型肺炎标本感染乳鼠和Vero E6细胞的病理学观察

PATHOLOGICAL OBSERVATION ON SUCKLING MICE AND VERO E6 CELLS INOCULATED WITH SARS SAMPLES

为查明非典型肺炎的病原体 ,作者以死亡患者肺组织感染KM乳鼠和VeroE6细胞 ,对发病鼠的主要脏器作病理学检查 ,对出现病变的培养细胞作超微结构观察 ,对细胞培养上清液作电镜负染检查。结果发现 ,患者肺组织可致乳鼠发病死亡 ,病变主要表现在肺脏和肝脏 ,在肺组织中检出病毒。在感染的培养细胞中发现大量病毒 ,在细胞培养液中也检出病毒 ,检出的病毒大多呈球形 ,直径 90～12 0nm ,其形态和细胞内分布与冠状病毒相一致 。

To identify the pathogen of severe acute respiratory syndrome (SARS ), suckling mice and Vero E6 cells were inoculated with the lung samples from autopsied patients with SARS. The tissues obtained from mice were examined with light microscopy and electron microscope(TEM). The infected cells and supernatants were also examined under TEM. The primary hiatopathological leisions in the lungs were wall of lung alveolies thickened and alveolar cavities narrowed. The hepatocytes were swollen and undergone vacuolar and hydropic degeneration. A large quantity of coronavirus like particles could be observed in the infected cells and supernatants and also in mouse pneumocytes under TEM. The viruses are round with diameters 80～120nm.The isolated coronavirus was associated with the current SARS epidemics.