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从尿中分离制备人表皮生长因子 被引量:1

Separation and preparation of human epidermal growth factor from urine
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摘要 目的探索从人尿中分离制备人表皮生长因子的工艺。方法通过超滤、蛋白质等电点沉淀、离子交换色谱和分子筛柱色谱等 ,从正常男性尿中分离、制备人表皮生长因子。结果从尿液 4 0L中 ,最后得到初步电泳纯 ,具有促细胞增殖活性的人表皮生长因子 2 .36mg。
出处 《中国生化药物杂志》 CAS CSCD 2003年第2期86-87,共2页 Chinese Journal of Biochemical Pharmaceutics
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参考文献6

  • 1王武康,朱劼.小鼠颌下腺表皮生长因子的制备及纯化[J].中国生化药物杂志,2000,21(6):295-296. 被引量:1
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二级参考文献3

  • 11,Cohen S.Isolation of a mouse submaxillary gland protein accelerating incisor eruption and eyelid opening in new-born animal.J Biol Chem,1962,237∶1555~1562
  • 22,Savage CR, Cohen S.Epidermal growth factor and a new derivative.J Biol Chem,1972,247∶7609~7611
  • 33,Taylor JM,Mitchell WM,Cohen S.Characterization of the high molecular weight form of epidermal growth factor.J Biol Chem,1974,249∶3198~3203

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