中国野生葡萄组织培养研究

Tissue culture of chinese wild grape

对中国野生山葡萄左山-1、左山-2、燕山葡萄燕山-1和秋葡萄平利-7的叶片、叶柄、茎段及单芽茎段进行了离体培养研究。诱导左山-1叶片分化出不定芽的培养基为MS+BA5.0mg/L+NAA0.1mg/L,诱导率2.5％;诱导平利-7叶柄分化出不定芽的培养基为MS+BA7.0mg/L+NAA0.1mg/L,诱导率1.95％;诱导左山-1、燕山-1和平利-7茎段分化出不定芽的培养基与叶柄相同,但诱导率相对较高,分别为8.25％、4.88％和6.49％;应用这一培养基对平利-7、左山-2的单芽茎段进行培养,丛状不定芽的诱导率均为100％。不定芽继代培养基为MS+BA0.5mg/L+IBA0.2mg/L;生根培养基为1/2MS+IBA0.1～0.2mg/L。

The leaf ,petiole, stem-segment and stem-segment with single bud of the chinese wild grapes,Zuoshan-1, Zuoshan-2 (V. amurensis),Yanshan-1 (V. yeshanensis) and Pingli-7 (V. romanetii) were in vitro cultured. The optimal medium for the leaf of Zuoshan-1 was MS+BA 5. 0 mg/L+NAA 0. 1 mg/L,and the inducing rate of adventitious buds was 2. 5%. The most effective medium for inducing adventitious buds from the petioles of Pingli-7 was MS+BA 7. 0 mg/L+NAA 0. l mg/L,and the inducing rate was 1. 95%. The optimum medium for the stem-segments inducing of Zuoshan-1,Yanshan-1 and Pinli-7 was MS+BA 7. 0 mg/L+NAA 0. 1 mg/L and the rate was 8. 25% ,4. 88% and 6. 49% respectively. Furthermore,over-grown adventitious buds were induced from stem-segments with single bud of Pingli-7,Zuoshan-2 in this medium and the percentage was all 100%. The subculture medium was MS+BA 0. 5 mg/L+IBA 0. 2 mg/ L. The rooting medium was 1/2 MS+IBA 0. 1-0. 2 mg/L.