高效液相色谱法测定脑血栓片中芍药苷的含量

Paeoniflorin Content in Naoxueshuan Tablet Determined by HPLC

目的:应用高效液相色谱法对脑血栓片中的芍药苷进行含量测定,从而建立脑血栓片的质量标准。方法:选用HypersilC_18分析柱(250mm×4.0mm,5μm),0.05mol/L磷酸二氢钾:甲醇(64:36)为流动相,检测波长为230nm,流速为1.0ml/min。结果:线性范围:0.4～2.4μg(r=0.9999)平均回收率为99.10％,RSD为0.78％。结论:本法简便、灵敏、准确。

To conduct content determination for paeoniflorin in Naoxueshuan Tablet by HPLC,thus set up the quality standard of Naoxueshuan Tablet.Method:Selecting Hypersil 18 an- alyzing column (250mm×4.0mm,5mm),0.05mol/L potassium dihydrogen phosphate:methanol (64:36)as mobile phase,Detection wavelength an 230nm;Flow rate at 1.0ml/min.Results:Lin- ear range;0.4～2.4mg(r=0.9999).Average recover rate at 99.01％,RSD at 0.78％.Conclusion: The method is simple and convenient,sensitive and accurate.