蓝萼香茶菜对大鼠全心缺血-再灌注时心肌c-fos基因表达的影响

Effects of Varglaucocalyx on c-fos Gene Expression during Globl Myocardial Ischemia-Reperfusion in Rat

目的 :研究蓝萼香茶菜对大鼠全心缺血 再灌注时心肌c fos基因表达的影响。方法 :采用Langen dorff离体全心缺血 再灌注损伤模型 ,Wistar大鼠 40只 ,随机分为 5组 ,每组 8只。N组为单纯K H液灌注对照组 ;CN组为缺血 再灌注对照组 ,(高XH ,中XM ,低XL浓度 )蓝萼香茶菜灌注 3组 (浓度分别为 5 % ,1 % ,0 .2 % )。采用免疫组化SP法 ,用相应的c fos抗体检测心肌细胞中c fos基因表达情况。用计算机图像分析系统分析c fos基因表达情况。结果 :与N组相比 ,CN ,XH ,XM ,XL组c fos基因表达水平明显提高 (P <0 .0 1 )。与CN组相比XH ,XM ,XL组c fos基因表达水平明显降低 (P <0 .0 5)。XM ,XL组较XH组c fos基因表达水平明显降低 (P <0 .0 1 )、XM较XL组相比c fos基因表达下降 (P <0 .0 5)。结论 :蓝萼香茶菜能显著抑制心肌c fos基因的表达 ,它保护缺血 再灌注心肌损伤可能部分与其抑制、下调c fos基因的蛋白表达有关。蓝萼香茶菜具有与剂量相关的心肌保护作用 ,但并非浓度越大越有效。

Objective:To detemine the effects of Varglaucocalyx on c fos gene expresson during global myocardial ischemia reperfusion. Method:Forty Wistar rats were divided into 5 groups: group N as control; group CN as ischemia reperfusion control and group XH, XM and XL treated with Varglaucocalyx 5%, 1%, 0.5% respectively prior to ischemia reperfusion. The isolated rat hearts were perfused in condition of constant temperature and pressure,and then the left ventricular myocardiums were extracted for use. The expression of c fos protein was detected by immunochemical method. The expression of c fos protein were quantified by using computer image analysis system. Result: Compared with the values of group N, protein expressions relative area of c fos gene(PERA)were increased significantly in group CN, XH, XM, XL( P <0.01), but decreased significantly in group XH, XM, XL, compared with those of group CN ( P <0.05) .The PERA of c fos gene in group XM, XL were significantly lower than in group XH( P <0.01), and the PERA of c fos gene in group XM were lower than in group XL( P <0.05). Conclusion: Varglaucocalyx can effectively depress the expression of c fos gene in myocardium which may account for its protection against myocardial ischemia reperfusion injury, and the middle and the low concentrations of Varglaucocalyx are more effective than the high concentrations.[