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麦胚凝集素修饰脂质体对小鼠口服吸收胰岛素的促进作用 被引量:5

The enhancing effect on insulin oral absorption of WGA modified liposomes in mice
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摘要 目的:研究麦胚凝集素修饰的脂质体对正常小鼠口服胰岛素的胃肠吸收促进作用。方法:采用碳化二亚胺交联法制备麦胚凝集素(WGA)修饰的磷脂酰乙醇胺(PE),将WGA-PE掺入胰岛素脂质体中制成凝集素修饰脂质体并证实WGA凝集活性不受影响。正常小鼠灌胃给予7 iu/只胰岛素修饰脂质体溶液,用葡萄糖酶试剂盒测定小鼠血糖,并与同剂量普通胰岛素脂质体比较。结果:麦胚凝集素修饰脂质体在6 h降糖百分率为(41.91±7.53)%.12 h达到(35.90±7.75)%,18 h为(61.13±4.00)%,维持降糖作用24 h。胰岛素普通脂质体几乎没有降糖作用,与生理盐水对照组相当。结论:麦胚凝集素修饰脂质体可能通过对细胞表面特异性受体的结合作用促进大分子药物的胃肠吸收。 AIM:The study was undertaken to investigate the enhancing effect on insulin absorption through GI. tract in mice by using the wheat germ agglutinin(WGA)modified liposimes as the carrier. METHOD: WGA modified phosphatidylethanolamine(PE)was synthesized by conjugating l-ethyl-3-(3'-dimethylaminopropyl) carbodiimide (EDC), then the modified compound (WGA-PE)was incorporated into the conventional liposome of insulin to obtain WGA modified liposome. The agglutination test was performed to examine the WGA biological activities after synthesis and modification. When liposomes were applied to the mice at insulin dose of 7 u/Kg orally, the hypoglycemic effect was investigated based on the blood level determination. RESULT:The blood glucose levels reduced by WGA modified liposomes were (41.91 ±7.53)% at 6h, (35.90 ± 7.75)% at 12 h, (61.13 ± 4.00)% at 18 h after oral administration respectively. The hypoglycemicaction remained up to 24 h. The conventional liposome and saline had not this effect. CONCLUSION: The WGA modified liposomes promote the oral absorption of insulin due to the specific-site combination on GI cell membrane.
出处 《中国天然药物》 SCIE CAS CSCD 2003年第1期30-33,共4页
基金 国家自然科学基金资助重点项目(No.39930200)
关键词 麦胚凝集素 修饰 脂质体 小鼠 胰岛素 促进作用 血糖 Insulin Wheat germ agglutinin Liposomes Blood glucose
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  • 1[1]Lehr CM.Lectin-mediated drug delivery:the second generation of bioadhesives[J]. J Control Rel,2000,65:19-29
  • 2[2]Haas J,Lehr CM.Developments in the area of bioadhsive drug delivery systems[J].Expert Opin Biol Ther,2002,2(3):287-298
  • 3[3]Childer NK, Denys FR, McGee NF,et al. Ultrastructral study of liposome uptake by M cells of rat Peyer's patch[J]. Immunol,1990,37:8-16
  • 4[4]Chen H,Torchilin V,Langer R.Lectin-bearing polymerized liposomes as potential oral vaccine carriers[J]. Pharm Res,1996,13(9):1378-1383
  • 5[5]Lambkin I,Pinilla C.Targgeting approaches to oral drug delivery[J]. Expert Opin Biol Ther, 2002,2(2):67-73

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