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严重急性呼吸综合征患者血清中存在抗肺组织抗体 被引量:8

There are autoantibodies against lung tissue in patients with severe acute respiratory syndrome
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摘要 目的 :建立特异性的检测严重急性呼吸综合征 (SARS)相关抗体的血清学方法。 方法 :制备 SARS患者肺组织包被抗原 ,采用酶联免疫吸附试验 (EL ISA)检测 SARS患者血清中特异性抗体。 结果 :SARS患者血清中存在 SARS相关的抗人肺组织抗体 ,其阳性率达 92 .86 %(2 6 / 2 8) ,正常献血员为 11.4 3%(12 / 10 5 ) ,统计学分析表明 ,SARS相关抗体的检出率在患者和献血员之间存在明显差异。 结论 :SARS患者血清中存在抗肺组织抗体 ,该抗体的存在提示机体免疫反应可能参与了疾病病理损伤过程 ;检测该抗体可能有助于 Objective: To set up a specific serological diagnosis method for severe acute respiratory syndrome(SARS). Methods: Specific antibodies were detected with enzyme linked immunosorbent assay (ELISA) using coated lung tissue as antigen and serum of SARS patients as antibodies.Results: Anti lung tissue antibodies were found in SARS patient serum, with the positive rate up to 92.86%(26/28);on the other hand, the positive rate of healthy blood donors was only 11.43%(12/105), and there was significant difference between SARS patients and healthy controls. Conclusion: There are some anti lung tissue antibodies in SARS patient serum, and the check of these antibodies will be helpful for diagnosis of SARS.The existence of autoantibodies may play a partial role in the SARS pathogenesis.
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出处 《第二军医大学学报》 CAS CSCD 北大核心 2003年第6期595-598,共4页 Academic Journal of Second Military Medical University
基金 国家自然科学基金科学部主任基金( 3 0 3 40 0 10 )
关键词 严重急性呼吸综合征 非典型肺炎 抗肺组织抗体 酶联免疫吸附试验 检测 血清学方法 severe acute respiratory syndrome autoantibodies lung enzyme linked immunosorbent assay
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