血管紧张素Ⅱ诱导乳鼠心肌细胞凋亡及牛磺酸的作用

Effects of Taurine on the Apoptosis of Neonatal Rat Cardiomyocytes Induced by AngiotensinⅡ

目的 观察外源性血管紧张素Ⅱ (AngⅡ )对心肌细胞凋亡的诱导作用 ,并探讨牛磺酸对其可能的作用和影响。方法 利用培养的乳鼠心肌细胞 ,随机分成不同AngⅡ浓度 (10 -9- 10 -5M )诱导的细胞凋亡组和不同浓度牛磺酸 (10、2 0、4 0mmol/L)作用组 ,观察培养 2 4h后的各组心肌细胞存活率 ,光镜和电镜观察凋亡细胞形态结构变化 ,TUNEL法检测凋亡 ,流式细胞仪检测细胞的凋亡率。结果 10 -9M的AngⅡ可诱导心肌细胞发生凋亡 ,心肌细胞呈现特征性的凋亡形态结构变化 ;TUNEL检测观察到凋亡细胞核呈黄褐色改变 ;流式细胞仪PI染色呈现典型的亚二倍体峰。牛磺酸可以明显的降低血管紧张素Ⅱ诱导的心肌细胞凋亡率 (P <0 .0 5 )。结论 一定浓度的AngⅡ可以引起体外培养的乳鼠心肌细胞发生调亡 。

Objective To investigate the effects and possible mechanism of taurine(Tau)on apoptosis in cultured myocytes of neonatal rat(NRCs)induced by Angiotensin (AngⅡ).Methods Cultured NRCs were randomly divided into four groups and were incubated with AngII at 10 -9 M and 10 -5 M for 24 h.Then cells were incubated with taurines and 10 -9 M Ang.The apoptosis of cells was detected by light microcopy,ransmission electron microscope,flow cytometery and TUNEL.Results The most obvious apoptosis of NRCs was found at the concertration of 10 -9 M of AngⅡ after 24 h of incubation.Taurine signicantly increased the survival rate of NRCs and attenuated apoptosis of NRCs induced by 10 -9 M of AngⅡ.Conclusion Angiotension II induce apoptosis of neonatal rat cardiomyocytes, while taurine could inhibit apoptosis.