SARS患者血清特异性IgG抗体的滴度变化

Variation in the Titer of the Specific IgG Antibody in Patients with SARS

目的了解严重急性呼吸道综合征(SARS)特异性IgG抗体滴度的变化。方法采用间接酶联免疫吸附试验(ELISA)检测10例SARS患者系列血清中特异性IgG抗体,系列血清包括患者发病后1,2,8,12w所采集的样本,通过血清稀释法测定抗体滴度。结果10例患者发病后第1周IgG抗体均为阴性;第2周时9例IgG抗体阳性,平均滴度为1∶40(临界值为1∶10);第8周时所有患者IgG抗体阳性,平均滴度为1∶256;第12周IgG抗体阳性平均滴度为1∶368。结论SARS特异性IgG抗体在急性后期产生,随病程滴度逐渐升高,其持续存在可能是获得病后免疫力的标志。

Objective To investigate the variation in the titer of SARS specific IgG antibody in patients with SARS(severe acute respiratory syndrome). Methods The titer of IgG antibodies against the SARS virus in the sera of the first week, second week, eighth week, twelfth week after onset of the illness in the 10 patients with SARS were determined by the indirect enzyme linked immunosorbent assay. Results Anti SARS virus IgG could not be detected in the first week after onset of clinical symptoms in all 10 patients. Of these 10 patients, 9 were sero positive in week 2 and at a low titer with an average of 1∶40 (positive cut off at 1∶10). All 10 patients were sero positive in week 8 with an average of 1∶256 and reached 1∶368 in week 12. Conclusion Anti SARS virus IgG antibody was found in most patients with SARS during the late acute or early convalescent phase after the onset of illness and the titer increased with the course of illness. The level of serum anti SARS virus IgG antibodies persisted for a long period after infection and might be protective and provide immunity from recurrence of symptomatic disease.