大鼠磨牙钻磨后牙髓中肌动蛋白表达的研究

Distribution of actin in burred rat dental pulps

目的 :探讨肌动蛋白在大鼠正常牙髓及钻磨刺激后牙髓中的分布变化。方法 :建立大鼠磨牙牙髓损伤模型 ,用免疫组化方法观察正常牙髓及钻磨后 2h～ 14d牙髓中肌动蛋白的分布情况。结果 :正常组成牙本质细胞及血管内皮细胞阳性 ;钻磨刺激 2h组 ,穿髓点附近的成牙本质细胞转为阴性 ,血管内皮细胞及管周细胞强阳性 ;8h～ 5d各组可见强阳性血管在牙髓中分布并始终位于炎症发展的前沿区。结论 :肌动蛋白对维持成牙本质细胞排列方式及正常生理功能有重要作用 ;其在血管内皮细胞及管周细胞的表达上调可能与牙髓损伤的早期修复有关。

Objective: To investigate the different distribution of actin in healthy and burred rat dental pulp.Method: Model of rat tooth injury was established to examine actin in the healthy pulp and the pulps from 2 hours to 14 days after burred by immunohistochemical technique.Result: In the healthy pulp, positive staining was localized in odontoblasts and vessel endothelia. 2h after injury, the odontoblasts near the exposure point turned negative while the vessel endothelia and their nearby cells were strongly labelled. Such strongly immunoreactive vessels always occurred in the area where pulpitis first attacked in the 8h～5d groups.Conclusion: Actin could play an important role in sustaining the odontoblasts in their specific lining style and physical function, and its up-regulation in vessels might have something to do with the early restoration of pulp after injury. [