病毒性肝炎患者lL-12及STNFR的检测及临床意义

Clinical significance of serum interleukin-12 and soluble tumor necrosis factor receptor in patients with viral hepatitis.

目的探讨病毒性肝炎患者血清中 IL-12与可溶性肿瘤坏死因子(sTNFR)的改变及其临床意义。方法双抗体夹心酶联免疫吸附法(ELISA)检测64例病毒性肝炎患者及20例正常对照血清 IL-12与sTNFR 水平。结果病毒性肝炎患者血清IL-12与 sFNFR 水平明显高于正常对照,与临床病型关系密切,在慢性肝炎、急性肝炎及重型肝炎依次升高,差异有显著性意义(P<0.01)。恢复期血清 IL-12水平迅速复常(P>0.05);血清 sTNFR 在急性肝炎恢复期,迅速复常(P>0.05),在慢性肝炎恢复期及部分重型肝炎恢复期血清 sTNFR 水平仍高于正常(P<0.05)。血清 IL-12与 sTNFR 水平与血清总胆红素及谷丙转氨酶水平呈正相关。结论检测血清 IL-12与 sTNFR 水平,可判断病情的严重程度及预后,指导临床诊断及治疗。

Objective To study the clinical significance of serum interleukin-12 (IL-12) and soluble tumor necrosis factor receptor (sTNFR) in patients with viral hepatitis.Methods The levels of IL-12 and sTNFR were measured by enzyme linked immunosorbent assay (ELISA) in 64 patients with viral hepatitis and 20 healthy adults subject as con- trois.Results The levels of IL-12 and sTNFR were significantly higher in patients with viral hepatitis than those in healthy controls.The variations of the serum IL-12 and sTNFR were closely associated with the clinical types of viral hepatitis.The IL-12 and sTNFR levels increased in the order chronic hepatitis (CH),acute hepatitis (AH) and se- vere hepatitis (SH) with significant difference between them and healthy controls (P<0.01).In convalescence.The serum IL-12 levels decreased to normal (P>0.05),The serum sTNFR levels decreased to normal in AH (P>0.05) and decrease to higher than normal in CH and in 4 cases of SH (P<0.05).The serum IL-12 and sTNFR levels were positively correlated with serum total bilirubin and alanine aminotransferase levels.Conclusion IL-12 and sTNFR participates in the activity process of viral hepatitis,but the effective elements of there is partial difference.Levels of se- rum IL-12 and sTNFR in early stage may reflect the severity of viral hepatitis and be helpful to prognosticating the dis- ease and guiding in clinical diagnosis and treatment.