盾叶薯蓣中薯蓣皂甙元的反相高效液相色谱测定

Determination of Diosgenin in Dioscorea Zingiberensis by Using Reversed-Phase High Performance Liquid Chromatography

<正> 盾叶薯蓣(Dioscorea Zingiberensis)为薯蓣科植物,其根茎俗称黄姜,产于四川、陕西、云南、湖南、湖北等省。本品为不常用中药,用于工业上提取皂素,主要成分是皂甙,水解得薯蓣皂甙元。它是异螺旋甾烷衍生物,化学名为Δ~5-异螺旋甾烯-3β-醇,是生产甾体激素类药物原料。以往测定薯蓣科植物中薯蓣皂甙元的含量多采用重量法、库伦法、薄层扫描等方法。这些定量方法操作繁杂,测定准确度不高。气相色谱测定皂甙元曾有文献报道。本文研究了薯蓣皂甙元的反相高效液相色谱测定。

This paper describes a reversed-phase high performance liquid chromatographic technique for quantitative determination of diosgenin in wild and cultivated dioscorea zingiberensis by the external stan- dardization method. The analytical results are satisfac- tory. In the cultivated dioscorea zingiberensis the contents of diosgenin are slightly higher than in the wild one.