摘要
应用地高辛甙元标记人乳头瘤病毒(HPV)-6,11,16和18探针,通过核酸杂交法检测上海地区女性患者HPV的感染。计活检组织标本128例,阴道脱落细胞130例,同时取样34例。斑点杂交结果表明活检组织中四种类型HPV的总阳性率:宫颈癌44%,宫颈间变66.7%,有间变史28.6%,外阴尖锐湿疣63%和慢性宫颈炎5.6%。阴道脱落细胞的各阳性率与之相近,同时取样的检测符合率为91.2%。Southern转移杂交结果提示:宫颈癌以HPV-16型为主。
Detection of human papillomavirus infection in female patients of the Shanghai area by nucleic acid hybridization uaing digoxigenin labelled HPV-6, 11, 16 and 18 probes was performed on 128 biopsy, 129 exfoliated vaginal cell and 34 both biopsy and exfoliated cell specimens. Positive rates combined HPV-6, 11, 16 and 18 in biopsy specimens of oervicrl carcinoma, cervical dysplasia, past cervical dysplasia, vaginal condylomata and cervitis were 44%, 66.7%, 28.6 %, 63% and 8.6% respectively by spot hybridization. Similar results were obtained on exfoliated cell specimens. In an analysis of 34 patients with both biopsy and exfoliated cell the coincident detection rate was 91.2%. Southern blot hybridization results showed that HPV in cervical carcinoma was mainly type 16, whereas in vaginal condylomata, types 6 and 11.
出处
《上海医科大学学报》
CSCD
1992年第4期289-293,共5页
Journal of Fudan University(Medical Science)
关键词
生殖道
核酸杂交法
乳头状瘤病毒
human papillomavirus
genital
digoxigenin
nucleic acid hybridization