父婴传播的乙型肝炎病毒S基因进化树分析

The analysis of S gene phylogenetic tree of HBV in transmission from father to infant

目的 分析研究乙型肝炎病毒 (HBV)父婴传播中父亲与新生儿所携HBV的基因进化树 ,从而证明HBV父婴传播途径的存在。方法 选择 16对父亲为HBV携带者而母亲无任何HBV感染标志的新生儿为研究对象 ,检测新生儿的HBV感染标志 ,将父亲与子代所携HBVS基因 45 1～ 6 6 0段序列作进化树分析。结果 16对父亲与新生儿所携HBV同属adw亚型 ,同源性 98%～ 10 0 %,检出HBVS区基因第 488、491、494、5 30、5 31、5 46、5 81、6 2 1位核苷酸变异 ,导致该区第 112、113、114、12 6、131、143、15 6位氨基酸替代 ,其中第 12 6位氨基酸由苏氨酸变为丙氨酸是基因库中所未见到的 ,该变异使该毒株在进化树位置上成为一新分支。结论 父婴传播中父亲与新生儿所携HBV同源性极高。我国人群HBV基因特点有不同与国外基因库中的位点。

Objective To study HBV transmission from father to infants, the study was carried out. Methods The study contained 16 pairs of fathers who was HBV carriers, and infants whose mothers wasn′t HBV carriers. The infants infected HBV in womb. The homogenous of HBV S gene were compared between fathers and infants, meanwhile HBV gene phylogenetic tree were analyzed. Results The genotype of 16 pairs fathers and infants were HBV adw. The homogenous of HBV S gene were 98%～100%.The mutation of 488、491、494、530、531、546、581、621 nucleotide of HBV S gene caused in112、 113、114、 126、131、143、156 amino acid substitution. The mutation of 126 amino acid from Threonine to Alanine didn′t existed in the gene bank. The sequence was difference from gene classification. Conclusion The homogenous of HBV S gene of fathers and infants was very high. The HBV gene characteristic in China was difference from gene bank.