日本血吸虫Mr=26×10^3谷胱甘肽S-转移酶基因植物表达载体的构建

Construction of Plant Expression Vector of Mr=26×103 Glutathione S-Transferase Gene of Schistosoma japonicum

【目的】构建日本血吸虫Mr=26×103谷胱甘肽S-转移酶(GST)基因的植物表达载体,为研制血吸虫病口服疫苗做前期准备。【方法】采用PCR技术,扩增目的基因GST,与植物表达载体pBI121连结,构建重组表达载体pBI121-GST。【结果】通过PCR检测和双酶切鉴定以及重组质粒序列测定,结果表明,该目的基因片段已被整合到植物表达载体pBI121中,通过电激转化,将质粒转入农杆菌菌株LBA4404、EHA105中。【结论】本实验成功地构建了日本血吸虫Mr=26×103谷胱甘肽S-转移酶GST基因的植物表达载体。

By construction of plant expression vector of Mr=26×103 Glutathione S transferase Gene of Schistosoma japonicum,to produce the oral vaccine for Schistosoma control. Mr=26×103 Glutathione S transferase Gene of Schistosoma japonicum was obtained by PCR. It was integrated into binary transformation vector: pBI121. The recombination plasmid pBI121 GST was identified by PCR amplification, restriction enzymes maps, and sequencing. It was transferred into agrobacterium tumefaciens strains LBA4404 and EHA105 by electrode methods. [Conclusion]Plant expression vector of Mr=26×103 Glutathione S transferase Gene of Schistosoma japonicum was successfully constructed in this experiment.