腾冲红花油茶蜜腺2个发育时期转录组差异性分析

Transcriptome Analysis of Camellia reticulata Nectary in Two Development Stages

【目的】植物蜜腺基因能够调控其分泌产物花蜜中的化学组成,从而影响植物对传粉者的吸引力,本研究主要揭示腾冲红花油茶蜜腺发育与分泌过程中其基因表达的变化情况。【方法】采用高通量Illumina Hiseq测序手段对花蕾期蜜腺与泌蜜高峰期蜜腺进行转录组测序,并对测序数据进行相关分析研究,探讨蜜腺发育与分泌过程中的调控基因。【结果】在蜜腺发育与分泌过程中共检测到58 488个差异基因,其中表达上调的基因有1 602个,表达下调的基因有693个。GO分析结果表明:被注释生物学过程的差异表达基因有3 096个,其中富集差异基因数量较多的生物学过程为胞内过程、生物调节过程和新陈代谢过程;富集于分子功能的差异表达基因有1 280个,其中富集差异基因数量较多的分子功能为连接功能、催化活性和载体活性。Pathway显著性富集分析发现:有2 147个差异表达基因参与了292条代谢途径,其中富集差异基因数量最多的途径是代谢途径(941个,占43.83%),主要是参与糖代谢、氨基酸代谢等过程。【结论】蜜腺发育及花蜜分泌过程中大多数差异表达基因与花蜜化学组成的调控过程有关。

[Purpose]The component of production secreted by nectary is regulated by the nectary gene. And the chemical component of nectar can directly affect the attraction for pollinator. This study was undertaken to reveal the change of gene expression profile of nectary in developing and secreting process.[Methods]The high-throughput Illumina Hiseq sequencing platform was used to investigate the transcriptome of nectary on the bud phase and nectary on the fifth day after anthesis.The sequencing data were subsequently analyzed to help reveal the change of gene expression profile in developing and secreting process.[Result]58 488 differentially expressed genes were detected in nectary developing and secreting process. After screening 1 602 up-regulated genes and 693 downregulated genes were obtained. Gene ontology enrichment analysis showed that 3 096 DEGs were involved in biological processes, the enrichment DEGs participated in biological regulation, cellular process and metabolic process. Meanwhile, 1 280 DEGs could be mapped as functional molecule, the enrichment DEGs involved in binding, catalytic activity and transporter activity. KEGG pathway enrichment analysis revealed that 2 147 DEGs were involved in 292 different metabolic pathways,which enriched larger numbers of DEGs including metabolism(941 genes, accounted for 43.83%),and it main involved in carbohydrate metabolism and amino acid metabolism.[Conclusion]The majority of differentially expressed genes in nectary developing and secreting process were related to the regulation of chemical component in nectar.