甘氨酸通过PTEN/AKT信号通路减轻大鼠脑出血损伤

Glycine Attenuates Intracerebral Hemorrhage in Rrats through PTEN/AKT Signaling Pathway

目的:探讨甘氨酸在大鼠实验性脑出血中的作用及其机制。方法:成年SD大鼠经左侧纹状体输注自体血100μL,造模后1 h侧脑室注射甘氨酸(0.2～3.0 mg/kg),甘氨酸注射前0.5 h注射Akt抑制剂Ⅳ(100μmol/L,2μL)。结果:脑出血早期血肿周围脑组织PTEN表达增加;甘氨酸降低PTEN表达,增加AKT磷酸化(Ser473)水平;甘氨酸治疗减少脑出血后死亡的神经元数量,减轻血脑屏障损伤及脑水肿,减小血肿面积,改善神经行为学评分;而Akt抑制剂Ⅳ部分阻断了甘氨酸的保护作用。结论:首次证实甘氨酸通过PTEN/Akt信号通路减轻大鼠脑出血损伤。

Objective To investigate the role and mechanism of glycine in experimental intracerebral hemorrhage(ICH)in rats.Methods Adult male SD rats were injected in 100μL autologous blood via the left striatum.Glycine(0.2~3.0 mg/kg)was injected into the lateral ventricle 1 hour after ICH,and Akt inhibitorⅣ(100μmol/L,2μL)was injected 0.5 hours before the injection of glycine.Results In the early stage of ICH,PTEN expression was up-regulated in brain tissue around hematoma;glycine decreased PTEN expression and increased AKT phosphorylation(Ser473);glycine treatment reduced neuronal death,blood-brain barrier damage,brain edema and hematoma area,and also improved the neurobehavioral score,while the Akt inhibitorⅣpartially blocked the neuroprotective effect of glycine.Conclusion Glycine was first demonstrated to play a protective role in ICH rats through PTEN/Akt signaling pathway.