摘要
分别从自然感染死亡的小鼠不同病理组织内分离到一种大小为250~300nm×160~190nm的卵圆型病毒粒子。以腹腔注射的方式将纯化的病毒粒子回复接种到小鼠体内,能引起小鼠死亡,从不同的病理组织内分离到与接种病毒粒子相同的病毒粒子。病理组织的超薄切片电镜观察结果表明在肝、肺、脾、肠等组织的细胞质内均能观察到病毒粒子的存在,表明病毒的复制和装配是在细胞质内完成的,该病毒应归类于痘病毒科。
A Mouse poxvirus has been isolated from different pathological tissues in deed mouse that natively infected by virus. In negative stained preparations, the size of the virus particles was 160~190nm×250~300nm and the virus the particle revealed ovoid-shape. The healthy Blab/C mouse were infected by injection with purified virus particles, resulting in the death of mouse and producing the same virus particles as inoculated virus. The electron microscopy of an ultrathin section confirmed that viral replication and assembly of virions occurred in the cytoplasm of different pathological tissues infected by viruses such as liver, lung, kidney, intestine and pancreas. These results demonstrated that the virus should been classified into Poxviridae.
出处
《中国病毒学》
CSCD
2003年第6期563-565,共3页
Virologica Sinica