摘要
目的 观察信号分子SonicHedgehog(Shh)及其受体Ptc1、Ptc2在小鼠下颌第一磨牙帽状期的基因表达 ,以探讨Shh信号路径在牙胚形态发育早期的作用。方法 制备昆明小鼠磨牙形态发育早期标本 (E 1 0 5~E 1 5 5) ,常规HE染色观察组织形态。免疫组化SP法对增殖细胞核抗原(proliferatingcellnuclearantigen ,PCNA)进行定位研究。原位杂交法分析Shh、Ptc1、Ptc2mRNA在牙胚中的表达与分布。结果 E 1 4 5 ,内、外釉上皮 ,星网状层及牙乳头细胞PCNA阳性 ,大部分釉结细胞PCNA阴性 ,少量釉结细胞PCNA阳性。Shh、Ptc1、Ptc2mRNA在内、外釉上皮、星网状层及釉结中均有表达。结论 在帽状期 ,信号分子Shh可能经自分泌或旁分泌途径作用于釉结以外的上皮和间充质 。
Objective To investigate the expression of Shh and its receptors Ptc1 and Ptc2 mRNA in the cap stage of mouse molar and discuss its role in early tooth morphogenesis Methods The embryonic mouse heads of early tooth development (E10 5~E15 5) were obtained and 5 μm serial sections were made Immunohistochemical staining of PCNA was carried out by SP method The expression pattern of Shh, Ptc1, and Ptc2 mRNA was analysed by in situ hybridization Results E14 5, outer dental epithelium ,inner dental epithelium ,stellate reticulum and underlying dental mesenchyme were PCNA positive Most of the enamel knot cells were PCNA negative A few of the enamel knot cells were PCNA positive Shh,Ptc1, and Ptc2 mRNA were strongly expressed in outer dental epithelium, inner dental epithelium , stellate reticulum and the enamel knot Conclusion In the cap stage, Shh as a paracrine and autocrine signaling molecule might stimulate epithelium and mesenchyme proliferation.
出处
《中华口腔医学杂志》
CAS
CSCD
北大核心
2003年第2期93-95,I002,共4页
Chinese Journal of Stomatology
基金
国家自然科学基金资助项目(301710 11)