期刊文献+

人胆囊上皮细胞炎症的PPAR-γ配体调控 被引量:3

Inflammatory regulation of human gallbladder epithelial cells by PPAR-γ ligand
下载PDF
导出
摘要 目的 :探索内皮细胞生长因子对胆囊上皮细胞培养的影响及噻格列酮对人胆囊上皮细胞炎症的调控 .方法 :培养基中添加内皮细胞生长因子进行细胞培养 ,将培养成功的胆囊上皮细胞分为对照组A1 ,A2 和试验组B1 ,B2 ,B3 ,B4,试验组及对照组A2 以促炎因子HIL β(人白细胞介素 1 β)制作胆囊上皮细胞炎症 ,各组IL 6值进行统计学分析 ,比较PPAR γ配体噻格列酮对试验组IL 6值的影响 .结果 :内皮细胞生长因子明显促进胆囊上皮细胞生长、延长体外存活时间 .试验组IL 6较对照组明显低 ,差别最大达 1 79.85pn·L-1 (P <0 .0 1 ) .结论 :内皮细胞生长因子有明显的促胆囊上皮细胞生长作用 ,PPAR γ配体噻格列酮能抑制胆囊上皮细胞炎症 。 AIM: To investigate the effect of endothelial growth factor (EGF) on human gallbladder epithelial cells culture and the inflammatory regulation of human gallbladder epithelial cells (HGBEC) by ciglitazone. METHODS: HGBE were cultured in medium with EGF and were grouped into the control groups and the test groups. HIL 1β were added into the test groups and the control group A 2 to make the inflammatory model of HGBEC. Media were collected to measure concentration of IL 6 by radio immunological analysis (RIA). The data were analysed statistically to study the effect of ciglitazone on IL 6. RESULTS: EGF prolonged the duration of HGBEC and improved the growth of HGBEC. IL 6 concentration in the test groups was more lower than that in the control groups ( P <0.01). CONCLUSION: EGF can improve the growth of HGBEC and ciglitazone can inhibit the inflammation of HGBEC, which may be the ideal alternative for treatment of acute and chronic cholecystitis.
出处 《第四军医大学学报》 北大核心 2003年第24期2224-2226,共3页 Journal of the Fourth Military Medical University
基金 教育部出国留学回国人员科研启动基金 [2 0 0 1 (345) ]
关键词 胆囊 上皮细胞 PPAR—γ 噻格列酮 炎症 gallbladder epithelial cells PPAR γ ciglitazone inflammation
  • 引文网络
  • 相关文献

参考文献2

二级参考文献5

共引文献16

同被引文献14

  • 1Qian Xie Kang-Da Liu Mei-Yu Hu Kang Zhou Experimental Research Center of Zhongshan Hospital,Fudan University,Shanghai,200032,China.SF/HGF-c-Met autocrine and paracrine promote metastasis of hepatocellular carcinoma[J].World Journal of Gastroenterology,2001,7(6):816-820. 被引量:24
  • 2程文,程南生,熊先泽,夏庆杰,陈清英,闫乃红.过氧化物酶体增殖物激活受体γ在肝门部胆管癌中的表达及其配体对胆管癌细胞生长的抑制作用[J].中华实验外科杂志,2005,22(11):1406-1406. 被引量:7
  • 3程文,程南生,熊先泽,夏庆杰,陈清英,闫乃红,敬静.PPAR-γ在肝门部胆管癌中的表达及其配体激活后对胆管癌细胞的作用[J].四川大学学报(医学版),2006,37(5):745-749. 被引量:4
  • 4Livak KJ,Schmittgen TD.Analysis of relative gene expression data using real-time quantitative PCR and the 2(delta-delta Ct)methoa[J].Methods,2001,25(4):402-408
  • 5Fajas L,Debril MB,Auwerx J.Peroxisme proxisome proliferators-activated receptor-gamma:From adipogenesis to carcinogenesis[J].J Mol Endocrinol,2001,27(1):1-9
  • 6Sunsmi E,Tsuno NH,Kitayama J,et al.Decreassed synthesis of metalloproteinase-7 and adhension to extracular matrix protein of human colon cancer cells treated with troglitazone[J].Surs Today,2002,32(4):343-350
  • 7Parsons SL,Swatson SA,Brown PD,et al.Matrix metalloproteinase[J].Br J Surg,1997,84(2):160-166
  • 8Ikenaka Y,Yoshiji H,Kuriyama S,et al.Tissue inhibitor of metalloproteinase-1(TIMP-1)inhibit tumor growth angiogenesis in TIMP-1 transgenic mouse model[J].Int J Cancer,2003,105(3):340-346
  • 9Coussens LM,Fingleton B,Matrisian LM.Matrix metalloprotelnase inhibitors and cancer:triais and tribulations[J].Science,2002,295(5564):2387-2392
  • 10Ozaki I,Mizuta T,Zhao G,et al.Involvement of the Ets-1 gene in overexpression of matrilysin in human hepatocellular cercinoma[J].Cancer Res,2000,60(22):6519-6525

引证文献3

;
使用帮助 返回顶部