摘要
目的 :探索内皮细胞生长因子对胆囊上皮细胞培养的影响及噻格列酮对人胆囊上皮细胞炎症的调控 .方法 :培养基中添加内皮细胞生长因子进行细胞培养 ,将培养成功的胆囊上皮细胞分为对照组A1 ,A2 和试验组B1 ,B2 ,B3 ,B4,试验组及对照组A2 以促炎因子HIL β(人白细胞介素 1 β)制作胆囊上皮细胞炎症 ,各组IL 6值进行统计学分析 ,比较PPAR γ配体噻格列酮对试验组IL 6值的影响 .结果 :内皮细胞生长因子明显促进胆囊上皮细胞生长、延长体外存活时间 .试验组IL 6较对照组明显低 ,差别最大达 1 79.85pn·L-1 (P <0 .0 1 ) .结论 :内皮细胞生长因子有明显的促胆囊上皮细胞生长作用 ,PPAR γ配体噻格列酮能抑制胆囊上皮细胞炎症 。
AIM: To investigate the effect of endothelial growth factor (EGF) on human gallbladder epithelial cells culture and the inflammatory regulation of human gallbladder epithelial cells (HGBEC) by ciglitazone. METHODS: HGBE were cultured in medium with EGF and were grouped into the control groups and the test groups. HIL 1β were added into the test groups and the control group A 2 to make the inflammatory model of HGBEC. Media were collected to measure concentration of IL 6 by radio immunological analysis (RIA). The data were analysed statistically to study the effect of ciglitazone on IL 6. RESULTS: EGF prolonged the duration of HGBEC and improved the growth of HGBEC. IL 6 concentration in the test groups was more lower than that in the control groups ( P <0.01). CONCLUSION: EGF can improve the growth of HGBEC and ciglitazone can inhibit the inflammation of HGBEC, which may be the ideal alternative for treatment of acute and chronic cholecystitis.
出处
《第四军医大学学报》
北大核心
2003年第24期2224-2226,共3页
Journal of the Fourth Military Medical University
基金
教育部出国留学回国人员科研启动基金 [2 0 0 1 (345) ]