哮喘小鼠肺组织Nuocytes增多与GITR-GITRL表达的关系

Relationship between increased nuocytes and GITR-GITRL expression levels in lung tissue of asthmatic mice

目的:检测哮喘小鼠肺组织中Nuocytes相关指标和GITR-GITRL的表达,探讨哮喘时Th2极化微环境的改变。方法:选择8只Balb/c小鼠,随机分为2组,模型组于第1天,第11天用OVA致敏,第22至26天用2%OVA溶液雾化激发,对照组用PBS处理;采用流式细胞术检测小鼠肺组织内GITR表达和Nuocytes细胞数量;qRT-PCR法检测Foxp3、RORα、ICOS、ST2、IL-5、IL-13和GITR及其配体GITRL的mRNA表达水平;对GITR-GITRL和Nuocytes及其相关分子的表达水平进行相关性分析。结果:与对照组相比,模型组小鼠肺组织中Nuocytes细胞数和GITR表达均升高,且GITRL、Foxp3、RORα、ICOS、ST2以及IL-5和IL-13 mRNA表达水平均明显上调;GITR与RORα和ST2、IL-5、IL-13的表达水平均呈正相关。结论:哮喘小鼠肺组织中Nuocytes和GITR-GITRL表达增高,提示哮喘时GITR-GITRL上调的T细胞免疫应答可能与Th2主导的免疫失衡和Nuocytes极化相关。

Objective: To detect the changes of numbers of nuocytes and levels of GITR-GITRL in the lung of asthmatic mice,analyze the relationship between them,then to explore the changes of Th2-polarized micro-environment at the locus of allergic inflammation when with asthma. Methods: Eight mice were devided into 2 groups randomly,asthma models were sensitized with OVA on 1st,11 st day,then nebulized inhalation with OVA on day 22-26,control group with PBS. Flow cytometry was used to detect numbers of nuocytes and cells which express GITR; qRT-PCR was also used to test the mRNA levels of GITR-GITRL,molecules and cytokines associated with nuocytes; pearson correlation analysis was used to detect the correlation between GITR-GITRL and nuocytes. Results: Compared with the control group,model group showed high levels of nuocytes and GITR-GITRL,meanwhile,with increased mRNA levels of GITRL,Foxp3,RORα,ICOS,ST2,IL-5 and IL-13; and there was a positive correlation between GITR expression and RORα,ST2,IL-5,IL-13 expression. Conclusion: Nuocytes and the expression of GITR-GITRL increased synchronously in lung of asthmatic mice,which suggested that the up-regulation of T cell-mediated immune response by GITR-GITRL may be associated with Th2-biased response and polarization of nuocytes.