Caspase-3与Bcl-2蛋白在膀胱移行细胞癌中的表达及意义

Expression of Caspase-3 and Bcl-2 in Bladder Transitional Carcinoma and Their Significance

背景和目的:肿瘤的发生是细胞异常增生和凋亡不足的结果。以往研究认为凋亡的发生与Caspase-3和Bcl-2蛋白有密切的联系。本研究拟通过检测Caspase-3和Bcl-2蛋白在膀胱移行细胞癌中的表达,探讨其在膀胱移行细胞癌发生、发展中的意义。方法:采用免疫组织化学(SP)法,对52例膀胱移行细胞癌组织和10例正常膀胱粘膜组织中的Caspase-3及Bcl-2蛋白表达情况进行检测。结果:膀胱移行细胞癌组织中的Caspase-3蛋白表达率(53.8%,28/52)明显低于正常膀胱粘膜组织(90.0%,9/10),Caspase-3的蛋白表达与膀胱移行细胞癌的病理分级有关(P<0.05),但与临床分期没有明显的联系;膀胱移行细胞癌组织中的Bcl-2蛋白表达率(51.9%,27/52)明显高于正常膀胱粘膜组织(20.0%,2/10),且Bcl-2蛋白表达情况与病理分级、临床分期均无关(P>0.05)。在正常膀胱粘膜中Caspase-3与Bcl-2的蛋白表达呈负相关(rs=-0.659,P<0.01)。结论:Caspase-3蛋白的高表达与Bcl-2蛋白的低表达在膀胱癌的发生和细胞凋亡的调控方面可能起着重要作用。

BACKGROUND &OBJECTIVE: Tumor growth was the result of cell excessive proliferation and lack of apoptosis. Apoptosis has been considered to be closely associated with Caspase 3 and Bcl 2. This study was designed to investigate the expression of Caspase 3 and Bcl 2 to evaluate their effects on the tumorigenesis and progression in bladder transitional carcinoma (BTCC). METHODS: The immunohistochemistry (SP method) was used to determine the expression of Caspase 3 and Bcl 2 in 52 cases of BTCC and 10 normal bladder mucosas. RESULTS: The protein expression level of Caspase 3 in BTCC (53.8%,28/52) was significantly lower than that in normal bladder samples (90.0%,9/10). Caspase 3 expression level was correlated with the grades of BTCC (P< 0.05), but had no significant correlation with clinical stages. The protein expression of Bcl 2 in BTCC (51.9%,27/52) was significantly higher than that of normal bladder samples (20.0%,2/10), while its expression level was not related to stage and grade of BTCC (P >0.05). The expression of Caspase 3 is negatively related to that of Bcl 2 (rs=-0.659,P< 0.01) in bladder tissues. CONCLUSION: Both over expression of Caspase 3 and descended expression of Bcl 2 may play an important role in the tumorigenesis and apoptotic regulation of bladder tissues.