急性白血病细胞中CD19的表达

Expression of CD19 in 321 cases of acute leukemia

目的 观察 CD19在急性白血病 (AL )中的表达与分布 ,为白血病的诊断、鉴别以及导向治疗提供依据。方法 采用 2 7个荧光直标单克隆抗体 (单抗 )及 CD45/ SSC双参数设门多色流式细胞术对 3 2 1例 AL细胞进行免疫诊断和分型 ,并对 CD19在各类型 AL细胞中的表达情况进行分析。结果 在 116例 B细胞系急性淋巴细胞性白血病 (AL L)患者中 ,CD19的阳性率 (99.1% )明显高于 B细胞系其它相关性抗原的阳性率 ;CD19在 17例含 B细胞系成分的杂合型白血病 (HAL )中全部表达 ,而在 2 9例 T细胞系 AL L和 7例 T/ My HAL则均无表达 ;在 152例急性髓系白血病 (AML)中 ,仅 11例 (7.2 % ) CD19阳性 ,明显低于其在 B细胞系 AL L 中的阳性率 (P=0 .0 0 1) ;CD2 2在 B/ My HAL的阳性率 (12 / 15,80 .0 % )明显高于 CD19+ -AML (0 / 11,0 % ,P<0 .0 0 1)。结论 CD19对 B系AL L 细胞的特异性较好 ,敏感性较高 ,是诊断 B细胞系 AL L 较为可靠的表面标记 ,也可作为导向治疗 B细胞系AL

Objective To investigate the expression and distribution of CD19 on acute leukemia(AL) cel ls and its significance. MethodsUsing a panel of 27 fluorochrome directly labeled monoclonal antibodies, samples from 321 AL patients were analy zed by CD45/SSC double parameters and multi-color flow cytometry to determine t he expression of CD19. ResultsIn 116 cases of B lineage ac ute lymphoblastic leukemia (ALL), the positive rate of CD19 (99.1%) was signific antly higher than that of the other B cell related antigens. CD19 was expressed on all 17 cases of B/myeloid (My) and B/T lineage hybrid acute leukemia (HAL), b ut was not expressed on 29 cases of T lineage leukemia and 7 cases of T/My HAL. In 152 cases of acute myeloid leukemia (AML), only 11 cases expressed CD19. The positive rate of CD19 (7.2%) on AML was significantly lower than that on B line age ALL (P=0 001). The positive rate of CD22 (12/15,80.0%) on B/My HAL was higher than that (0/11,0%,P<0 001) on CD19 +-AML. ConclusionThe sensitivity and specificity of CD19 on B lineage ALL are high, CD19 is continuously and stably expressed on all stages of B lineage differentiation. I t might be a reliable cell membrane marker for diagnosing B lineage ALL and an ideal target for antibody-targeting treatment of leukemia as well.