期刊文献+

三种自动化免疫分析系统测定HBV血清标志物结果比较 被引量:52

Comparison of the results by three automatic immunoassay for determination of HBV markers in serum
下载PDF
导出
摘要 目的 探讨不同的自动化免疫分析系统检测乙肝病毒血清标志物结果的一致性。方法 采用AxSYM、Elecsys 2 0 1 0和ARCHITECTi2 0 0 0三种自动化免疫分析系统分别对 1 2 0例随机选择的住院患者血清标本进行乙肝表面抗原 (HBsAg)及其抗体 (抗 HBs)、乙肝核心抗体 (抗 HBc)、乙肝e抗原 (HBeAg)及其抗体 (抗 HBe)等五项血清标志物测定 ,并以AxSYM结果为参照 ,计算三种方法所获结果的符合率。结果 对 1 2 0例随机血清标本 ,Elecsys 2 0 1 0与AxSYM比较测定结果符合率分别为HBsAg 99.2 %、抗 HBs 85 .8%、抗 HBc 87.5 %、HBeAg 99.2 %、抗 HBe 90 .0 % ;ARCHITECTi2 0 0 0与AxSYM比较测定结果符合率分别为HBsAg 1 0 0 % ,抗 HBs96 .7%、抗 HBc 91 .7%、HBeAg1 0 0 %以及抗 HBe95 8%。Elecsys 2 0 1 0与AxSYM测定抗 HBs的相关系数r =0 .90 ,ARCHITECTi2 0 0 0与AxSYMr =0 .98。Elecsys 2 0 1 0与AxSYM测定HBV五项标志物模式符合率为 6 5 .8% ,ARCHITECTi2 0 0 0与AxSYM测定五项标志物模式符合率为 85 .0 %。结论 三种方法测定HBV结果有一定差异 ,主要存在于三项抗体标志物以及低浓度抗原标志物的测定 。 Objective To discuss the agreement of three automatic immunoassay systems for determining HBV markers in serum. Methods A total of 120 serum samples were collected randomly from hospitalized patients determined for five HBV markers including HBsAg, Anti HBs, Anti HBc, HBeAg and Anti HBe by AxSYM, Elecsys 2010 and ARCHITECT i2000,respectively .The concordance rates by Elecsys 2010 and ARCHITECT i2000 were calculated and compared with that by AxSYM.Results The concordance rates (%) by Elecsys 2010 with AxSYM was 99.2% for HBsAg, 85.8% for Anti HBs, 87.5% for Anti HBc, 99.2% for HBeAg and 90.0% for Anti HBe, respectively,and the concordance rate by ARCHITECT i2000 with AxSYM was 100% for HBsAg, 96.7% for Anti HBs,91 7% for Anti HBc, 100% for HBeAg and 95.8% for Anti HBe, respectively.The correlation coefficient (r) of Elecsys 2010 with AxSYM was 0.90 for Anti HBs, lower than that of ARCHITECT i2000 with AxSYM( r =0.98).The concordance rate by Elecsys 2010 for the five HBV markers was 65.8% and that of ARCHITECT i2000 was 85.0%.Conclusion There were some discrepant results among three automated immunoassay systems for determining HBV markers in serum,mostly in HBV specific antibodies and low level antigens.The determining methods should be standardized furtherly.
出处 《国外医学(临床生物化学与检验学分册)》 2004年第1期3-5,共3页 Foreign Medical Sciences(section of Clinical Biochemistry and Laboratory Medicine
关键词 自动化免疫分析系统 测定 HBV血清标志物 免疫分析 对比研究 HBV markers immunoassay comparative study
  • 引文网络
  • 相关文献

参考文献6

  • 1杨守纯,陶其敏.我国病毒性肝炎免疫学检测史的回顾与展望[J].中华医学检验杂志,1999,22(5):275-277. 被引量:42
  • 2陈瑜,钟步云,徐根云,王磊,刘招娣,单谷忠,马锦洪.低水平血清乙肝病毒表面抗原测定及其临床意义[J].中华检验医学杂志,2001,24(1):39-41. 被引量:155
  • 3Heijtink RA,Schneeberger PM,Postma B,et al.Anti-HBs levels after hepatitis B immunisation depend on test reagents: routinely determined 10 and 100IU/L seroprotection levels unreliable.Vaccine,2002,20: 2899-2905.
  • 4Weber B,Dengler T,Berger A,et al.Evaluation of two new automated assays for hepatitis B virus surface antigen (HBsAg) detection: IMMULITE HBsAg and IMMULITE 2000 HBsAg.J Clin Microbiol,2003,41:135-143.
  • 5Weber B,Melchior W,Gehrke R,et al.Hepatitis B virus markers in Anti-HBc only positive individuals.J Med Virol,2001,64:312-319.
  • 6骆抗先.乙型肝炎的基础与临床:第2版[M].北京:人民卫生出版社,2001.237-240.

二级参考文献21

  • 1侯金林,骆抗先,梁炽森,王战会.HBsAg阴性乙型肝炎病毒感染与S基因插入变异的关系[J].中华传染病杂志,1996,14(3):129-133. 被引量:41
  • 2杨守纯,解放军医学杂志,1982年,7卷,217页
  • 3Tao Q M,Chin Med J,1980年,93卷,415页
  • 4杨守纯,中华医学检验杂志,1978年,1卷,13页
  • 5骆抗先,第一军医大学学报,1998年,18卷,87页
  • 6祁自柏,中华医学检验杂志,1998年,21卷,348页
  • 7周继文,中华医学检验杂志,1998年,21卷,30页
  • 8成军,现代肝炎病毒分子生物学,1997年,267页
  • 9骆抗先,乙型肝炎.基础与临床,1997年,143页
  • 10周育森,军事医学科学院院刊,1996年,20卷,249页

共引文献186

同被引文献298

引证文献52

二级引证文献241

;
使用帮助 返回顶部