银杏萜内酯的分离、纯化和结构鉴定

Isolation,Purification and Identification of Ginkgo Terpene Lactones

目的 :研究银杏 (GinkgobilobaL .)叶中的萜内酯类成分。方法 :95 %乙醇提取 ,索氏提取分段 ,硅胶柱层析得到总内酯 ,再用制备型RP HPLC方法得到纯化合物 ,用IR、1HNMR、13 CNMR ,尤其是通过 2DNMR光谱技术确定化合物结构。结果 :分得 6个萜内酯类成分 ,分别为白果内酯 (Ⅰ )、银杏内酯A(Ⅱ )、银杏内酯B(Ⅲ )、银杏内酯C(Ⅳ )、银杏内酯J(Ⅴ )和银杏内酯K(Ⅵ )。结论 :其中化合物Ⅵ为新化合物 ,并通过 2DNMR光谱技术对所分得的银杏内酯类化合物的碳氢信号进行了确切归属。银杏内酯K在 10 -6～ 10 -5mol/L的终浓度下 ,可对 2× 10 -7mol/LPAF刺激的中性粒细胞β 葡萄糖酸苷酶的释放有明显的抑制作用。

AIM:To isolate and elucidate terpene lactones from the leaves of Ginkgo biloba L. METHOD:Chromatography on silica gel and preparative RP HPLC were used. The structure was determined by IR, MS, 1HNMR , 13 CNMR , especially 2D NMR experiments.RESULT:Six terpene lactones were isolated, purified and elucidated as bilobalide(Ⅰ), ginkgolide A(Ⅱ), ginkgolide B(Ⅲ),ginkgolide C(Ⅳ), ginkgolide J(Ⅴ) and ginkgolide K(Ⅵ) respectively.CONCLUSION:Compound Ⅵ is a new compound.