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绿茶提取物对1,2-二甲基肼诱发小鼠大肠隐窝上皮细胞微核与凋谢的拮抗作用 被引量:4

THE ANTAGONISTIC EFFECTS OF GREEN TEA EXTRACT ON MICRONUCLEI AND APOPTOSIS INDUCED BY 1,2-DIMETHYLHYDRAZINE(1,2-DMH) IN THE COLON CRYPT CELLS OF MICE
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摘要 绿茶多酚和绿茶水溶性提取物对1,2-二甲基肼(1,2-DMH)诱发近交系C_(57)BL小鼠大肠隐窝上皮细胞微核与凋谢的影响研究结果显示:提取物的不同剂量对1,2-DMH诱发小鼠大肠隐窝上皮细胞微核和凋谢有明显的拮抗作用,并存在剂量-效应关系。因而测定二甲基肼诱发小鼠大肠隐窝上皮细胞微核和凋谢,可作为抗结肠致癌物的快速筛选方法。 Thrity-six inbr.ed C57 BL mice were randomly divided into 12 groups (3 mice each). Group 1 to 6 assigned to GTP (green tea polyphenols) groups and other 6 groups to GTW (green tea water extract) groups. In GTP groups, mice of group 1 drank tap water in the whole period of experiment, group 2,3,4 were given GTP solution (1 mg/mouse, 2mg/mouse, 4mg/mouse, respectively) once a day for five days. Mice of group 5 and 6 were given distilled water and GTP solution (4mg/mouse) respectively orice a day for five days. On the fifth day, 20mg 1,2-DMH/kg body weight was intraperitone-ally administered to the mice of group 1,2,3 and 4, whereas group 5 and 6 received 1 mmol EDTA solution (0.5ml/mouse) as controls.In GTW groups, treatment was similar to that of GTP groups except the different doses of GTW (2mg/mouse, 4mg/mouse, 8mg/mousc, respectively).The mice were killed at 24 hours after intraperitoneal injection of 1,2-DMH. The results indicated that the frequencies of micronuclei and apop-tosis in the colon crypt cells of mice were negatively correlated with the doses of GTP or GTW. Green tea extract had distinct antagonistic effects on micronuclei and apoptosis in the mouse colon crypt cells. The assay of micronuclei and apoptosis in the colon crypt cells of mice may be a simple, rapid, economical and useful test as a screening method for anticolon carcinogens.
出处 《营养学报》 CAS CSCD 北大核心 1992年第3期255-259,共5页 Acta Nutrimenta Sinica
基金 河南省卫生厅资助
关键词 微核 凋谢 绿茶 提取物 致癌物 green tea extract micronuclei apoptosis anticolon-carcinogen
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