急性胆道感染和胆道梗阻时肝脏枯否细胞吞噬功能和有效肝脏血流量的实验研究

An experimental study on Kupffer cell phagocytic function and effective hepatic blood flow in acute obstructive cholangitis and biliary obstruction

研究了大鼠急性胆道感染(AOC)和胆道梗阻(BDL)时祜否细胞(KC)吞噬功能和有效肝脏血流量(EHBF)的改变,发现:KC吞噬功能在AOC组2h明显增强,并持续至48h,在BDL组48h见有明显增强;EHBF在AOC组2h明显降低,6h逐渐恢复至正常,48h又见有明显升高,在BDL组24h明显降低,持续至48h。提示:EHBF的改变与KC吞噬功能的改变无相关性,KC吞噬功能增强可能是机体对细菌或内毒素的快速防御机制之一,严重胆道梗阻早期可能常常伴有内毒素血症的发生。

The changes of Kupffer cell (KC) phagocytic capacity (KCPC) and effective hepatic blood flow (EHBF) in acute obstructive cholangitis (AOC) and bile duct ligation (BDL)were studied in rats. The results showed that KCPC (T_(50) min) was significantly increased at AOC 2h (9.3±0.3 versus 4.2±0.4, P<0.01). In BDL group, a significant increase was seen at 48h (10.1±0.2 versus 7.9±0.9, P<0.05). EHBF (T_(50) min) was significantly decreased at AOC 2h (4.3±0.1 versus 5.9+±0.3, P<0.05) and returned to normal at 6 h and got increased at 48h (3.8±0.7 versus 2.3+±0.2, P< 0,01), And EHBF Was decreased siginificantly at BDL 24h (3.9±0.2 versus 5.1±0.6, P<0.05) and further decreased at 48h (3.8±0.7 versus 6.7±0.8, P<0.01). These results indicated that there was no significant correlation between EHBF and KCPC. The increase of KCPC might be one of the rapid defensive mechanisms of the body against invading bacteria or endotoxins. Endotoxemia may exist in the early stage of severe biliary obstruction.