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P物质与神经微丝H在慢性痛血管球瘤中的表达和意义 被引量:7

Expression and significance of substance P,neurofilament-H in glomus tumors with chronic pain
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摘要 目的 观察P物质 (substanceP ,SP)、神经微丝 H(neurofilament H ,NFH)在慢性痛血管球瘤中的表达和分布 ,探讨其在血管球瘤慢性痛的形成及其与疼痛程度的关系。 方法  2 7例慢性痛血管球瘤组织标本分为轻症组 (12例 ) ,重症组 (15例 ) ,与 30例富含正常血管球的手指甲根组织对照(对照组 ,30例 ) ,采用免疫组织化学方法和计算机图像定量分析技术对SP、NFH的表达进行观察和检测。 结果 SP、NFH在 3组内均有表达 ,以重症组表达最强烈 [灰度值SP(14 3 3± 7 5 ) ,灰度值NFH(16 7 7± 4 4 ) ],轻症组表达其次 [灰度值SP(15 6 2± 8 2 ) ,灰度值NFH(194 8± 4 0 ) ],对照组表达最弱[灰度值SP(2 0 8 2± 16 6 ) ,灰度值NFH(2 2 5 1± 8 3) ],3组间差异有非常显著意义 [重症组与轻症组 (PSP=0 0 0 2 ,PNFH<0 0 0 0 1) ,重症组与对照组 (PSP <0 0 0 0 1,PNFH <0 0 0 0 1) ,轻症组与对照组 (PSP <0 0 0 0 1,PNFH<0 0 0 0 1) ];病例组SP、NFH表达的定量灰度值与疼痛分数分别呈高度负相关 (rsp=-0 8974 ,Psp=0 0 0 0 0 0 1;rNFH=- 0 6 5 4 5 ,PNFH=0 0 0 0 2 12 )。 结论 SP是血管球瘤慢性痛形成过程中的外周主要疼痛传入递质 ,NFH与疼痛递质的传递活动密切相关。 Objective To observe the expression and distribution of substance P(SP), neurofilament-H(NFH) in glomus tumors with chronic pain,and to discuss the process of chronic pain and the relationship with pain degree. Methods Twenty-seven patients diagnosed as glomus tumor with chronic pain were enrolled as case group, and divided into light pain symptomatic group (LPSG)(n=12) and severe pain symptomatic group (SPSG)(n=15) according to clinical manifestations. Control group(CG)were enrolled by 30 patients with amputated extremities or hands after trauma. Immunohistochemical methods were used to determine the expression of SP,NFH which were detected quantitatively by computer graph analysis system too. Results The positive expression and distribution of SP, NFH existed in all the three groups and SPSG expression level was the highest [Grayscale Value SP(143.3±7.5),Grayscale Value NFH(167.7±4.4)], LPSG followed [Grayscale Value SP(156.2±8.2),Grayscale Value NFH(194.8±4.0)], control group was the third[Grayscale Value SP(208.2±16.6), Grayscale Value NFH(225.1±8.3)];The difference of expression level among three groups was significant [SPSG vs LPSG(P SP=0.002,P NFH<0.0001),SPSG vs CG(P SP<0.0001,P NFH<0.0001),LPSG vs CG(P SP<0.0001,P NFH<0.0001)]. The findings of Pearson product-moment correlation analysis between quantitative grayscale value of SP,NFH respectively and pain score in all the patients with glomus tumor showed linear negative correlation (r SP=-0.8974,P SP=0.000001; r NFH=-0.6545,P NFH=0.000212). Conclusion SP is the mainly afferent pain transmitter in the process of chronic pain in glomus tumor, and NFH plays an important role in pain-transmitted activities.
出处 《中华外科杂志》 CAS CSCD 北大核心 2003年第12期935-939,共5页 Chinese Journal of Surgery
关键词 P物质 神经微丝H 慢性痛血管球瘤 表达 Glomus tumor Pain Substauce P Immunohistochemistry
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