维生素C、E对汞急性肾毒作用影响的实验研究

Experimental study on effects of vitamin C and vitamin E on the acute nephrotoxicity of mercury

目的 探讨VitC和VitE对汞急性肾毒作用的影响。方法 Wistar大鼠随机分成 6组 :对照组 ,低、中、高剂量染汞组 ,VitC、VitE预处理组。染毒 12h后收集 12h尿样 ,采集血液 ,分离血清 ,切取肝脏、肾皮质样品。测定肝、肾皮质和尿中汞含量 ,尿N 乙酰 β D氨基葡萄糖苷酶 (NAG)、碱性磷酸酶 (ALP)、乳酸脱氢酶 (LDH)活性和尿蛋白、血清尿素氮 (BUN)含量。结果 高剂量染汞组肝、肾皮质和尿中汞含量显著高于对照组 ,且肾皮质中汞含量显著高于肝汞 ,高剂量染汞组尿NAG、ALP、LDH活性和尿蛋白、BUN含量显著高于对照组。VitC和VitE预处理组肝、肾皮质和尿中汞含量显著高于对照组 ,尿NAG、ALP活性和尿蛋白含量显著低于高剂量染汞组 ,LDH活性和BUN含量也低于高剂量染汞组 ,且VitC预处理组与高剂量染汞组差异有显著性。

Objective To study the effects of Vit C and Vit E on the acute nephrotoxicity caused by mercury.Method Wistar rats were divided into four groups randomly,i.e.,control group,Hg exposed group(including mild exposure,middle exposure and high exposure three subgroups),Vit C treatment group and Vit E treatment group.At 12th hour after last mercury exposure,the animals were held in metabolic cages for 12 hours,collected the urine,liver,renal cortex and blood samples for the measurement of Hg contents in liver,renal cortex and urine;the urinary activities of N-acetyl-β-D-glucosaminidase(NAG),alkaline phosphatase(ALP),lactic dehydrogenase(LDH);the urinary levels of protein and the blood urea nitrogen(BUN).Result The Hg contents of liver,renal cortex and urine,the urinary activities of NAG,ALP,LDH,the urinary level of protein and BUN content in high exposure subgroup were all significantly higher than that of control group,and the Hg content in renal cortex was higher than that in liver.The results also showed that the Hg contents in liver,renal cortex and urine in Vit C group and Vit E group were all significantly increased as comparing with those of control,while the urinary activities of NAG,ALP and LDH,the urinary protein level and BUN in Vit C and Vit E groups were also significantly lower than that of high exposure subgroup.Conclusion Vit C and Vit E have an antagonistic effect on the acute nephrotoxicity of mercury.