摘要
目的 建立大鼠血浆中龙胆苦苷的检测方法。方法 采用RP—HPLC法测定大鼠血浆中药物浓度 ,色谱柱为LichrospherC18(4 .6× 2 5 0mm ,5 μm) ,流动相为甲醇 -水 (33:6 7V/V)。 结果 在 1~ 2 0mg .L- 1范围内龙胆苦苷峰面积与浓度线性关系良好 (r =0 .9997) ,最低可定量浓度为 0 .0 3mg .L- 1,绝对回收率为 88.4 %~ 96 .2 % ,准确度为 97.8%~ 10 4 .9% ,日内、日间RSD均小于 10 %。结论 本测定方法灵敏、准确、简便 ,适合于龙胆苦苷的药代动力学研究。
Aim To develop a simple,sensitive and rapid method for the determination of gentiopicroside in rat′s plasma. Method The plasma concentration of gentiopicroside was determined by RP-HPLC method ,the analysis involved a 250mm × 4.6mm column packed with C 18 (5um),the mobile phase consisted of methyl alcohol-water(33∶67) Result A good linearity was obtained in the range of 1~20 mg. l-1 (r=0.9997),the recovery was between 88.4~96.2%, the accuracy was between 97. 8%~104.9% .The relative stand deviations of within-day and between day were less than 10%,the minimal detectable concentration in rat′s plasma was 0.03 mg. l-1 Conclusion The result demonstrated that the method was sensitive,accurate and simple for the determination of gentiopicroside in rat′s plasma,it is suitable for the pharmacokinetics study of gentiopicroside.
出处
《青海医学院学报》
CAS
2004年第1期12-14,共3页
Journal of Qinghai Medical College