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以P33重组蛋白为诊断抗原建立牛瑟氏泰勒虫病乳胶凝集试验 被引量:11

Latex Agglutination Test of Theileriosis Sergenti by P33 Recombinant Protein
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摘要 本试验用P33重组蛋白建立了牛瑟氏泰勒虫病乳胶凝集试验。对81份被检血清的检测结果,阳性率为44.4%,明显高于血涂片镜检法的21.0%(P<0.01),而且在该病流行地区的不同牧场、不同性别和不同年龄牛的阳性率之间未见显著差异。试验表明,以P33重组蛋白作为诊断抗原具有特异性强、抗原易纯化和成本低等优点。 Latex agglutination test of Theileriosis sergenti based on P33 recombinant protein was established first time. Our results suggested that positive rate of ox blood serum was 44.4% by this method. 81 ox blood serum specimens from different pasture, different sex and different age were detected, and didn't found significant discrepancy in positive rate between them. Meanwhile ox blood serum positive rate was 21.0%(P<0.01) by blood smear with microscopic examination. The present study indicates that the assay is characterized by its specificity, simplicity and economical cost.
出处 《畜牧兽医学报》 CAS CSCD 北大核心 2004年第3期347-349,共3页 ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金 牛瑟氏泰勒虫病的免疫学研究(中韩合作项目)。
关键词 瑟氏泰勒虫病 乳胶凝集试验 P33重组蛋白 诊断抗原 P33 recombinant protein Latex agglutination test Theileriosis sergenti
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参考文献7

  • 1Kawazu S, Kamio T, Kakuda T, et al. Phylogenetic relationships of the benign Theileria species in cattle and Asian buffalo based on the major piroplasm surface protein (p33.34) gene sequences[J]. Int J Parasitol,1999,29: 613~618.
  • 2Kang S W, Kweon C H, Choi E J,et al. Expression of major piroplasm protein (p33) of Theileria sergenti (Korean isolate) and its immunogenicity in guinea pigs[J]. Korean J Parasitol,1999,37(4): 277-283.
  • 3Kang S W, Choi E J, Kweon C H. Cloning and sequencing of p33 in a Korean isolate of Theileria sergenti[J].Korean J Parasitol,1997,35(2): 105-110.
  • 4Kawazu S, Okumura T, Hirogari Y,et al. A polymorphism observed in the experimentally successful peptide vaccine sequence derived from Theileria sergenti piroplasm major surface antigen (p33) [J]. J Vet Med Sci 1997,59(9): 829~831.
  • 5许应天,张守发,李顺玉,金明虎.牛泰勒虫病的诊断及预防研究进展[J].延边大学农学学报,1997,19(4):271-275. 被引量:27
  • 6殷宏,张其才,吕文祥,窦惠芳,吕文顺.补体结合试验诊断环形泰勒虫病的研究[J].中国兽医科技,1996,26(7):9-11. 被引量:9
  • 7吴鉴三,张肖正,周立桥,梁建波,王新,王树双,丁江.奶牛瑟氏泰勒虫病诊断和综合防制的研究[J].中国兽医科技,1996,26(6):16-17. 被引量:3

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