雷公藤多甙对哮喘患者痰液IL-5和嗜酸细胞凋亡的影响

Effects of Tripterygium polyglucosideo (TP) on cytokines and eosinophil apoptosis in asthmatic patients in sputum

目的 探讨雷公藤多甙 (TP)对哮喘患者的治疗作用。方法 选择 70例轻、中度哮喘缓解期患者 ,随机分成A组 (TP治疗组 )和B组 (对照组 ) ,治疗前后分别采用ELISA法测定患者痰液白细胞介素 5 (IL 5 )和可溶性白细胞介素 2受体 (sIL 2R) ,应用流式细胞仪检测痰液嗜酸细胞 (EOS)凋亡。结果 缓解期哮喘患者痰液中IL 5和sIL 2R明显升高。A组经TP治疗后 ,IL 5和sIL 2R明显降低 ,EOS凋亡率明显升高 ,B组治疗前后上述各项指标差异无显著性。结论 TP可能通过抑制哮喘患者T细胞和B细胞的活化 ,增加EOS的凋亡 ,从而减少机体免疫 炎症反应。

Objective To explore the mechanism of anti-inflammatory and anti-asthmatic action of TP in asthmatic patients. Methods Seventy asthmatic patients at stable stage were randomly divided into group A (TP-treated group) and group B (control group). IL-5 and sIL-2R in sputum were determined by ELISA, eosinophil (EOS) apoptosis was identified by flow cytometer. Results IL-5 and sIL-2R in sputum were significantly increased, and IL-5 and sIL-2R in sputum were decreased and EOS apoptosis percentages were significantly increased in group A after TP-treatment, and there was no significant difference among the above parameters in group B before and after treatment. Conclusion TP inhibited T and B cell activation and increased EOS apoptosis in asthmatic patients, and thus reduced immune-inflammation of the patients, the bronchus can be dilated and thus lung function improved.