多孔聚氨酯固定化黑曲霉产β-葡萄糖苷酶条件的研究

Study on Immobilization of Aspergillus niger for β-Glucosidase Production

以多孔聚氨酯材料为固定化载体 ,就固定化培养条件中的孢子浓度、pH值、温度、固液比等进行考察 ,经正交试验优化后的最佳固定化条件为固液比为 1 / 90、孢子浓度为 1 0 5mL-1 、温度为 2 8℃ ,pH值 4 .5 ,固定化条件中影响黑曲霉产 β 葡萄糖苷酶的因素显著性顺序为固液比 >孢子浓度 >pH值 >温度。与游离态菌丝体相比较 ,固定化细胞发酵产酶的持续稳定性和活力都有所提高 ,固定化细胞酶产量达 1 30 .0 0u/mL。

Immobilization conditions of mycelia of Aspergillus niger to be absorbed within porous polyurethane as carrier materials were studied through single factor orthogonal experiments. The optimized conditions are solid/liquid ratio at 1/90, spore concentration at 105ml{-1}, 28℃,pH 4.5. The order of factors that conspicuously affect βglucosidase production are solid/liquid ratio > spore concentration > pH > temperature. As compared with mobile mycelia, immobilized one is more continuously stable and raise its activity up to the βglucosidase production at 130.00μ/ml.;