脾虚患者血浆细胞因子变化及与脾虚证关系的研究

Study on the Relationship between the Change of Plasma Cytokines of Patients with Spleen Deficiency and the Syndrome of Spleen Deficiency

目的:观察脾虚患者血浆细胞因子的变化,探讨脾虚证与细胞因子的关系。方法:68例脾虚证患者随机分为治疗组(四君子汤治疗)和对照组,每组34例。采用免疫分析技术测定两组血小板活化因子(PAF)、转化生长因子β_1(TGF-β_1),白三烯C_4(LTC_4)的含量。并与34例健康献血员(正常对照组)对照。结果:试验第30天,脾虚证对照组血浆PAF、LTC_4的含量比四君子汤治疗组含量明显高,血浆TGF-β_1的含量比四君子汤治疗组的含量明显低。经过四君子汤治疗后,脾虚患者TGF-β_1、PAF、LTC_4的含量恢复正常。结论:TGF-β_1、PAF、LTC_4与脾虚证有关。

To observe the change of plasma cytokines of patients with spleen deficiency and discuss the relationship between spleen deficiency and cytokines. Methods:Sixty-eight patients with spleen deficiency were randomly assigned to Sijunzi decoction treatment(SDT, n=34) group and spleen deficiency(SD, n=34) group. The levels of plasma platelet activating factor(PAF), transforming growth factor-β_1 (TGF-β_1), leukotriene C4 (LTC_4) were measured with radioimmunoassay respectively. Results:The levels of PAF and LTC_4 of SD group were higher than those of SDT group while the level of TGF-β_1 of SD group was lower than that of SDT group on the 30 th day. After the treatment with Sijunzi decoction,the levels of PAF, TGF-β_1 and LTC_4 of the patients with spleen deficiency group were normalized. Conclusior: The levels of PAF, TGF-β_1 and LTC_4 are associated with spleen deficiency syndrome.