TNF-α在小鼠心肌应激性损伤中表达的实验研究

An experimental study on the expression of TNF-α in myocardial injury during stress

目的 :探讨TNF -α在应激性心肌损伤中的表达和意义。方法 :以异丙肾上腺素 (isoproterenol,ISO)作腹腔注射 ,建立小鼠应激性心肌损伤模型 ,应用免疫组化SP方法检测TNF -α在小鼠心肌中的表达。结果 :TNF -α在正常对照组偶见于局部视野的心肌细胞及血管内皮细胞 (VEC) ;TNF -α在应激组的表达大大增加 ,且增加的程度与心肌的损害程度一致。结论 :TNF -α在应激性心肌损伤中的表达明显增加 ,其增加程度与心肌损伤的程度密切相关 ,提示TNF

Objective:To detect TNF-α in the tissue of myocardium injury during stress and discuss its significance.Methods:To establish a model of myocardium lesion by injecting ISO to mice abdominal cavity,then TNF-α in the tissue of heart was assessed by immunohistochemical method of SP and HE staining in paraffin sections.Results:There were little positive substance of TNF-α expressed in heart in normal control.The positive substance of TNF-α increased significantly after stress.Conclusion:Increase in the expression of TNF-α in stress indicate its participation in the process of myocardium injury,and it may be an objective marker for evaluation of myocardium injury.