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NK细胞促进小鼠MHC半相合骨髓移植后造血重建的实验研究 被引量:6

Experimental study on NK cells promoting donor marrow engraftment and hematopoietic reconstitution after MHC haploidentical BMT in mice
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摘要 目的 探讨将纯化的供鼠NK细胞加入预处理方案对主要组织相容性复合物 (MHC)半相合小鼠骨髓移植 (BMT)后造血重建的影响。方法 以BALB/cH 2d×C5 7BL/ 6 H 2b(CB6F1 H 2d/b)小鼠为受者 ,以C5 7BL/ 6 H 2b小鼠为供者。 5 0只受鼠经 9.0Gy致死剂量照射后随机分为 5组 ,每组 1 0只。①对照组 :分别为单纯照射组、MHC半相合BMT组。②实验组 :CB6F1 H 2d/b小鼠在完成照射后 1h内首先经尾静脉输注纯化后的MHC半相合供者NK细胞 ,分为 1× 1 0 6/只、5× 1 0 5/只、2× 1 0 5/只 3组 ,在照射后 4h移植MHC半相合骨髓细胞。以血常规、体重、生存时间、病理组织学等变化为观察指标和进行组间比较。结果 ①生存期 :单纯照射对照组为 (5 .1 5± 0 .6 6 )d ,其他组小鼠生存期均 >30d。②移植后第 1 0天时白细胞及血小板计数 :MHC半相合BMT对照组为 (0 .99± 0 .2 2 )× 1 0 9/L、(6 1 .0 0± 7.2 7)× 1 0 9/L。实验组中 3个不同NK细胞输注剂量组白细胞及血小板计数分别为 (2 .0 1± 0 .2 1 )× 1 0 9/L、(1 0 1 .5 0± 1 6 .34)× 1 0 9/L ;(1 .98± 0 .2 9)× 1 0 9/L、(99.5 0± 1 6 .4 1 )× 1 0 9/L ;(1 .97± 0 .2 1 )× 1 0 9/L、(98.0 0± 1 6 .1 9)× 1 0 9/L ,同对照组比较差异有显著性 (P <0 .0 1 )。组? Objective To explore the effect of donor derived NK cells added to pretreatment conditioning regimen on hematopoietic reconstitution after MHC haplotype mismatched BMT in mice. Methods Murine model of MHC haplotype mismatched BMT was established by using BALB/c H 2d ×C57BL/6 H 2b (CB6F 1 H 2d/b ) mouse as recipient, and C57BL/6 H 2b mouse as donor. Fifty recipient mice were divided into 5 groups . The mice in the first three groups were each infused 1×10 6 、5×10 5 、2×10 5 /mouse donor derived NK cells, respectively before TBI ( 60 Co, 9.0 Gy) and then conditioned with TBI, followed by infusion of C57BL/6 H 2b mice bone marrow cells four hours later. The mice in the fourth group received TBI only, and in the fifth group, TBI and BMT at the some doses as the first three groups. Hematopoietic reconstitution, survival time, body weight, histopathology of the recipients were followed up. Results ① Survival time was (5.15±0.66) days for the fourth group, and >30 days for the other 4 groups. ② Leukocyte and platelet counts at day 10 after BMT were (0.99± 0.22 )× 10 9 /L and (61.0±7.27)× 10 9 /L respectively for the fifth group and (2.01±0.21)× 10 9 /L,( 101.50 ±16.34)× 10 9 /L; (1.98±0.29)× 10 9 /L,(99.50±16.41)× 10 9 /L and (1.97±0.21)× 10 9 /L,(98.0±16.19)× 10 9 /L for the first three groups, respectively. Histopathology displayed no GVHD in all the groups. Conclusion Donor derived NK cells could promote hematopoietic reconstitution after MHC haplotype mismatched BMT in mice.
出处 《中华血液学杂志》 CAS CSCD 北大核心 2004年第5期290-292,共3页 Chinese Journal of Hematology
关键词 NK细胞 小鼠 MHC 骨髓移植 造血功能 病理组织学 Killer cells,natural Major histologic compatible complex Hematopoietic stem cell transplantation Hematopoietic reconstitution Mice
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