运动性动情周期抑制的大鼠下丘脑、垂体、血浆β—EP和性激素的变化

Changes of B-EP and Sex Hormone in Hypothalamus Pituitary and Plasma of Rats Under Athletic Menstrual Cycle Irregularities

在建立递增负荷训练的运动性闭经动物模型的基础上 ,采用放射性免疫法 ,测定 β—EP和性激素 ,观察不同负荷训练对大鼠下丘脑、垂体、血浆 β—EP和血浆T、FSH、LH、P、E2 的影响 ;AMI与 β—EP和性激素的关系。结果显示 ,长期大强度负荷使训练组A动情周期抑制 ,表现为低促性腺类固醇、低促性腺激素 ,而下丘脑、血浆 β—EP含量明显升高。短期适宜强度负荷使训练组C血T明显升高 ,长期大强度负荷使训练组A血T明显下降。经 1周休息后 ,β—EP、P、T、FSH已基本恢复 ,但LH、E2 未完全恢复。提示 :β—EP对下丘脑GnRH释放具有直接抑制效应。血T升高可直接对抗雌激素或负反馈作用于下丘脑—垂体性腺轴继发AMI。AMI经及时合理适当调整 ,大部分性激素是可以逆转的。

On the basis of establishing a rat model similar to athletic menstrual irregularities by incremental training and using the radio immunoassay (RIA) to determine β-EP and sex hormone so as to investigate 1. the effects of training at various loads on the items of β-EP in hypothalamus, pituitary and plasma and the items of plasma T, FSH, P, E 2 in rats; 2. the relationship between AMI, β-EP and sex hormone. The results show that the estrous cycle of rats in training group A is suppressed by long-term, heavy load training, that is, gonadotropic steroid and gonadotrophin decreases whereas the items of β-EP in hypothalamus and plasma increases significantly. After a week rest, the items of β-EP, P, T and FSH are restored while the items of LH and E 2 are not completely restored. The study gives the conclusion: 1. β-EP directly suppresses the release of GnRH in hypothalamus. 2. The increase of serum T directly abrogates E 2 or reacts against hypothalamus-pituitary-ovarium axis to form secondary AMI. 3. If AMI is properly modulated, most sex hormones may be reserved.