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DDRT-PCR Analysis of Wheat Roots Under Iron-Deficient Condition and Differential Expression of ABC Gene 被引量:2

DDRT-PCR Analysis of Wheat Roots Under Iron-Deficient Condition and Differential Expression of ABC Gene
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摘要 Differential expression of gene in iron-efficient wheat cultivar Jing411 and iron-inefficient cul-tivar SanshumaiS under iron-deficiency and iron-sufficiency conditions was revealed by differential display reverse transcript PCR (DDRT-PCR) method. Northern blotting was carried out using ATP-binding transporter (ABC) cDNA obtained from DDRT-PCR products of the cultivar Jing411 as probe. Our results suggested that ABC gene expression was suppressed under iron-deficiency condition. Differential expression of gene in iron-efficient wheat cultivar Jing411 and iron-inefficient cul-tivar SanshumaiS under iron-deficiency and iron-sufficiency conditions was revealed by differential display reverse transcript PCR (DDRT-PCR) method. Northern blotting was carried out using ATP-binding transporter (ABC) cDNA obtained from DDRT-PCR products of the cultivar Jing411 as probe. Our results suggested that ABC gene expression was suppressed under iron-deficiency condition.
出处 《Agricultural Sciences in China》 CAS CSCD 2002年第12期1317-1320,共4页 中国农业科学(英文版)
关键词 WHEAT ROOTS Iron nutrition DDRT-PCR Gene expression Wheat, Roots, Iron nutrition, DDRT-PCR, Gene expression
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  • 1Liang P,Nucl Acid Res,1993年,21卷,14期,3269页

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