期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

桂枝茯苓丸对荷瘤鼠Survivin、PCNA、P21^(waf/cip)的影响 被引量：4

Effects of guizhi fuling wan on survivin,P21waf/cip,and proliferating cell nuclear antigen of cancer-bearing mouse
下载PDF
导出
摘要 目的探讨桂枝茯苓丸抑瘤作用的分子机制,为桂枝茯苓丸(GFW)开发应用提供实验依据。方法计算抑瘤率,免疫组化法测定PCNA、P21waf/cip蛋白表达,原位杂交法检测Survivin mRNA表达。结果GFW抑瘤率为38.93%,GFW上调肿瘤细胞P21waf/cip表达,下调PCNA及Survivin表达。结论GFW可抑制肿瘤细胞生长,其机制可能与上调P21waf/cip及下调PCNA及Survivin mRNA表达密切相关。 Objective To Study the molecular mechanism of guizhi fuling wan(GFW) in controlling the tumor growth for providing experiment evidence of GFW developing and utilizing.Methods The S180 mouse sarcoma model was used for detecting the cancer inhibiting effects of GFW.The protein expression of proliferating cell nuclear antigen(PCNA),P21waf/cip,and the mRNA expression of survivin were evaluated by immunohistochemistry and in situ hybridization.Results For S180 sarcoma,the inhibiting ratio of GFW was 38.93%.Survi...
作者 王琪 罗晓庆 王慧 王亚贤
机构地区 齐齐哈尔医学院免疫教研室 黑龙江中医药大学微生物学与免疫学教研室
出处 《免疫学杂志》 CAS CSCD 北大核心 2009年第4期382-384,共3页 Immunological Journal
基金 黑龙江省教育厅资助课题(11511458)
关键词 桂枝茯苓丸 SURVIVIN PCNA P21waf/cip guizhi fuling wan survivin proliferating cell nuclear antigen P21waf/cip
分类号 R285.5 [医药卫生—中药学]
  • 相关文献

参考文献4

二级参考文献38

  • 1[6]Evans GI, et al. Proliferation: Cell cycle and apoptosis in cancer. Nature, 2001, 411: 342
  • 2Blangy A, Lane H A, d'Herin P, et al. Phosphorylation by p34cdc2 regulates spindle association of human Eg5, a kinesin-related motor essential for bipolar spindle formation in vivo. Cell, 1995, 83:1159～1169.
  • 3Nigg E A. Mitotic kinases as regulators of cell division and its checkpoints. Nat Rev Mol Cell Biol, 2001, 2:21～32.
  • 4Ando K, Ozaki T, Yamamoto H, et al. Polo-like kinase 1(Plk1) inhibits p53 function by physical interaction and phosphorylation. J Biol Chem, 2004, 279:25549～25561.
  • 5Myer D L, Bahassi el M, Stambrook P J. The Plk3-Cdc25circuit. Oncogene, 2005, 24:299～305.
  • 6Walsh S, Margolis S S, Kornbluth S. Phosphorylation of the cyclin b 1 cytoplasmic retention sequence by mitogen-activated protein kinase and Plx. Mol Cancer Res, 2003, 1:280～289.
  • 7Sherr C J, Roberts J M. Living with or without cyclins and cyclin-dependent kinases. Genes Dev, 2004, 18:2699～2711.
  • 8Brehrn A, Miska E A, McCance D J, et al. Retinoblastoma protein recruits histone deacetylase to repress transcription.Nature, 1998, 391:597～601.
  • 9Zhao J, Kennedy B K, Lawrence B D, et al. NPAT links cyclin E-Cdk2 to the regulation of replication-dependent histone gene transcription. Genes Dev, 2000, 14:2283～2297.
  • 10Xie S, Xie B, Lee M Y, et al. Regulation of cell cycle checkpoints by polo-like kinases. Oncogene, 2005, 24:277～286.

共引文献111

同被引文献49

引证文献4

二级引证文献22

免疫学杂志
2009年 第4期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部