摘要
Theo-aminophenol (OAP)-H2O2-horseradish peroxidase (HRP) voltammetric enzyme-linked immunoassay new system has extremely high sensitivity. HRP can be measured with a detection limit of 6.0×1010 g/L and a linear range of 1.0×10-9-4.0×10-6 g/L. The pure product of H2O2 oxidizing OAP catalyzed by HRP was prepared with chemical method. The enzyme-catalyzed reaction has been investigated with electroanalytical chemistry, UV/Vis spectrum, IR spectrum,13C NMR,1H NMR, mass spectrum, elemental analysis, etc. Under the selected enzyme-catalyzed reaction conditions, the oxidation product of OAP with H2O2 catalyzed by HRP is 2-aminophenoxazine-3-one. The processes of the enzyme-catalyzed reaction and the electroreduction of the product of the enzymecatalyzed reaction have been described.
The o-aminophenol （OAP）-H<sub>2</sub>O<sub>2</sub>-horseradish peroxidase （HRP） voltammetric enzyme-linked immunoassay new system has extremely high sensitivity. HRP can be measured with a detection limit of 6.0×10<sup>-</sup>（10） g/L and a linear range of 1.0×10<sup>-9</sup>—4.0×10<sup>-6</sup> g/L. The pure product of H<sub>2</sub>O<sub>2</sub> oxidizing OAP catalyzed by HRP was prepared with chemical method. The enzyme-catalyzed reaction has been investigated with electroanalytical chemistry, UV/Vis spectrum, IR spectrum, <sup>13</sup>C NMR, <sup>1</sup>H NMR, mass spectrum, elemental analysis, etc. Under the selected enzyme-catalyzed reaction conditions, the oxidation product of OAP with H<sub>2</sub><sub>O</sub>2 catalyzed by HRP is 2-aminophe-noxazine-3-one. The processes of the enzyme-catalyzed reaction and the electroreduction of the product of the enzymecatalyzed reaction have been described.
基金
Project supported by the National Natural Science Foundation of China (Grant No. 29775012)
